[Processing of clonogenic findings in limited dilution. Application to the quantification of autograft depletion]

Limiting dilution culture is a powerful tool for the quantification of residual clonogenic cells in the evaluation of a depletion procedure in bone marrow purging. The definition of suitable numerical indicators and the choice of the statistical technique for data treatment are of crucial importance. Most common techniques are presented and discussed. A standardization for data treatment is proposed.