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Updated: Dec 11, 2025

Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells
Published on: May 12, 2023
Two-pore channels as master regulators of membrane trafficking and endocytic well-being
Kristin Vassileva1,2, Mark Marsh1, Sandip Patel2
1MRC Laboratory for Molecular Cell Biology, University College London, London, UK.
Abstract:
Two-pore channels (TPCs) are a ubiquitous class of Ca2+- and Na+-permeable ion channels expressed within the endo-lysosomal system. They have emerged as central regulators of a wide array of physiological processes intimately linked to information processing. In this short review, we highlight how molecular and chemical strategies have uncovered multiple roles for TPCs in regulating various aspects of endo-lysosomal trafficking associated with disease. We summarise advances in the identification of new small molecules to pharmacologically target TPCs for medical benefit. Lastly, we discuss possible underpinning molecular mechanism(s) that translate TPC-mediated ionic fluxes to function.
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