Fluidic embedding of additional macroporosity in alginate-gelatin composite structure for biomimetic application

Biomimetic characteristics of hydrogel scaffold are tuned in this study utilizing the synergy of alginate, gelatin, and microfluidically embedded voids. Superposition of alginate and gelatin polymer networks results in additional rigidity, which can be tuned by introduction of voids, and thereby allowing faster release of pore pressure through movement of aqueous phase through the pore network. More importantly, voids enabled the cells to penetrate from the surface of seeding into the depth of the scaffold and proliferate there, as demonstrated for MDA MB 231 breast cancer cells. The uniform voids, generated by the microfluidic device, self-align creating uniform macroporosity within the gel structure, get readily filled by the media due to hydrophilicity, and extend the characteristics of composite uniformly across the entire scaffold.