Related Experiment Video

Updated: Dec 11, 2025

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
14:40

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

3.6K

N6-methyladenosine regulates PEDV replication and host gene expression

Jianing Chen1, Li Jin1, Zemei Wang1

  • 1State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, 730046, China.

Virology
|August 26, 2020
PubMed

Insights

RNA methylation, specifically N6-methyladenosine (m6A), plays a key role in porcine epidemic diarrhea virus (PEDV) infection. This study reveals m6A regulates viral replication and host responses.

Area of Science:

  • Virology
  • Epigenetics
  • Molecular Biology

Background:

  • N6-methyladenosine (m6A) is a crucial RNA modification impacting cellular processes.
  • The role of m6A in viral infections is an emerging area of research.
  • Porcine epidemic diarrhea virus (PEDV) is a significant pathogen in swine.

Purpose of the Study:

  • To investigate the impact of m6A RNA modification on PEDV replication.
  • To elucidate the roles of host methyltransferases and demethylases in PEDV infection.
  • To understand how m6A affects host responses during PEDV infection.

Main Methods:

  • Investigated m6A abundance in PEDV RNA and host cells.
  • Utilized knockdown of methyltransferases (METTL3, METTL14) and demethylase (FTO).
  • Analyzed the effect of YTHDF proteins on m6A-modified viral RNA stability.
  • Assessed changes in host RNA m6A sites and gene expression.

Main Results:

  • m6A modification is abundant in the PEDV genome.
  • METTL3/METTL14 knockdown enhanced PEDV replication; FTO silencing decreased viral output.
  • YTHDF proteins modulated PEDV replication via viral RNA stability.
  • PEDV infection increased host RNA m6A and decreased FTO expression.
  • Host responses to PEDV were influenced by m6A, reversible by FTO.

Conclusions:

  • m6A modification is integral to PEDV replication.
  • Host m6A machinery (METTL3, METTL14, FTO, YTHDF proteins) significantly influences PEDV.
  • PEDV manipulates host m6A levels to affect viral replication and host immunity.
Keywords:
Anti-Viral mechanismCoronavirusN6-methyladenosinePorcine epidemic diarrhea virusPosttranscriptional modification

More Related Videos

In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells08:58

In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells

Published on: May 1, 2019

15.5K
Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells10:22

Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells

Published on: November 12, 2015

9.0K

Related Concept Videos

Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.6K
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
33.1K
RNA Editing02:23

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.6K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.2K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

3.7K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
25.3K