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Function study of vasoactive intestinal peptide on chick embryonic bone development.

Liu Shi1, Chaojie Wang2, Yu Yan2

  • 1Department of Orthopaedics & Traumatology, Stem Cells and Regenerative Medicine Laboratory, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong SAR, PR China; Trauma Center, Zhongda Hospital, School of Medicine, Southeast University, No. 87 Ding Jia Qiao, Nanjing, PR China; School of Medicine, Southeast University, No. 87 Ding Jia Qiao, Nanjing, PR China.

Neuropeptides
|August 26, 2020
PubMed
Summary
This summary is machine-generated.

Vasoactive intestinal peptide (VIP) is crucial for embryonic bone development and innervation. This study shows VIP can reverse nerve damage-induced bone growth inhibition in chick embryos.

Keywords:
Chick embryoInnervationOsteogenesisVasoactive intestinal peptide

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Area of Science:

  • Developmental Biology
  • Neuroscience
  • Skeletal Biology

Background:

  • Embryonic bone development involves complex neuro-osteogenic interactions.
  • Vasoactive intestinal peptide (VIP) has diverse biological roles, including neurotransmission.
  • VIP's specific function in skeletal innervation and early bone development requires further elucidation.

Purpose of the Study:

  • To investigate the role of VIP in embryonic skeletal innervation and bone development using a chick embryo model.
  • To determine if VIP influences osteogenesis and bone formation during embryogenesis.

Main Methods:

  • Confirmed peripheral nerve fiber innervation in chick limb bone tissue using neurofilament (NF) and TUJ-1 markers.
  • Quantified VIP mRNA and peptide expression during embryonic bone development.
  • Utilized a chemical sympathectomy model (6-OHDA) to assess neurotoxin effects on bone development and VIP expression.
  • Evaluated VIP's ability to rescue 6-OHDA-induced inhibition of osteogenesis and bone development.

Main Results:

  • Innervation of limb bone tissue by peripheral nerve fibers was confirmed during embryonic development.
  • VIP expression levels increased in bone tissue with advancing innervation.
  • Chemical sympathectomy (6-OHDA) reduced neural crest formation, NF expression, and inhibited distal limb bone development and VIP expression.
  • Co-administration of VIP with 6-OHDA successfully rescued osteogenesis and bone development impairments.

Conclusions:

  • VIP plays a significant role in early embryonic bone development and innervation.
  • VIP can counteract the inhibitory effects of chemical sympathectomy on osteogenesis and bone development in chick embryos.