Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Glomerular Filtration01:15

Glomerular Filtration

4.2K
The filtration membrane in the renal system is a highly specialized structure essential for filtering blood. It consists of glomerular capillaries and podocytes, forming a selective barrier that permits the passage of water and small solutes while restricting most plasma proteins and blood cells.
Components of the Filtration Membrane
The filtration process involves three key layers: the glomerular endothelial cells, the basement membrane, and the podocyte-formed filtration slits.
4.2K
Renal Corpuscle01:20

Renal Corpuscle

6.3K
The glomerulus and Bowman's capsule are two essential components of the nephron, which is the functional unit of the kidney. These microscopic structures play a critical role in the process of blood filtration to produce urine.
Glomerulus: Structure and Function
The glomerulus is a tiny, intricate network of capillaries located at the beginning of the nephron. It's enveloped by the Bowman's capsule and receives its blood supply from an afferent arteriole, which divides into numerous...
6.3K
Basal Lamina are the Specialized Form of ECM01:03

Basal Lamina are the Specialized Form of ECM

3.5K
The basal lamina is a thin extracellular layer that lies underneath the cells and separates them from other tissues. The three layers of the basal lamina are lamina lucida, lamina densa and lamina reticularis. The basal lamina, a mixture of glycoproteins and collagen, provides an attachment site for the epithelium, separating it from underlying connective tissue. The framework of basal lamina has other essential proteins such as laminins mesh, perlecan, entactin, and type IV collagen.
Proteins...
3.5K
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

8.6K
The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
8.6K
Type IV Collagen of Basal Lamina01:05

Type IV Collagen of Basal Lamina

2.8K
Type IV collagen is a 400 nm long, network-forming collagen that acts as a barrier between the epithelial and endothelial cells. Type IV collagen  forms the backbone of the basement membrane by scaffolding with laminin, entactin, proteoglycans, and fibronectin. Apart from rendering structural support to the basement membrane, it also helps entail signaling potentials necessary for both pathological and physiological functions.
A type IV collagen molecule has six alpha chains which can...
2.8K
Integrins01:10

Integrins

4.9K
Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
4.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gene-disease relationships for glomerular phenotypes: expert recommendations from ClinGen.

Nature reviews. Nephrology·2026
Same author

Alport: Renaming an Extended Clinical Spectrum.

Journal of the American Society of Nephrology : JASN·2026
Same author

Integrin α 1 β 1 Promotes Interstitial Fibrosis and Cyst Growth in a Mouse Model of Polycystic Kidney Disease.

Journal of the American Society of Nephrology : JASN·2026
Same author

Increased <i>in vivo</i> transduction of AAV-9 cargo in Alport podocytes.

Wellcome open research·2025
Same author

Basement membranes at a glance.

Journal of cell science·2025
Same author

Balancing YAP and TAZ for Podocyte Survival.

Journal of the American Society of Nephrology : JASN·2025

Related Experiment Video

Updated: Dec 11, 2025

Highly Sensitive Measurement of Glomerular Permeability in Mice with Fluorescein Isothiocyanate-polysucrose 70
09:16

Highly Sensitive Measurement of Glomerular Permeability in Mice with Fluorescein Isothiocyanate-polysucrose 70

Published on: August 9, 2019

7.2K

Complexities of the glomerular basement membrane.

Richard W Naylor1, Mychel R P T Morais1,2, Rachel Lennon3,4

  • 1Wellcome Centre for Cell-Matrix Research, Division of Cell-Matrix Biology and Regenerative Medicine, School of Biological Sciences, Faculty of Biology Medicine and Health, The University of Manchester, Manchester Academic Health Science Centre, Manchester, UK.

Nature Reviews. Nephrology
|August 26, 2020
PubMed
Summary

The glomerular basement membrane (GBM) is vital for kidney filtration. Advances reveal its complexity and role in diseases like Alport syndrome, paving the way for new GBM repair therapies.

More Related Videos

Assessment of Kidney Function in Mouse Models of Glomerular Disease
09:16

Assessment of Kidney Function in Mouse Models of Glomerular Disease

Published on: June 30, 2018

18.3K
Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins
09:12

Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins

Published on: January 18, 2019

9.8K

Related Experiment Videos

Last Updated: Dec 11, 2025

Highly Sensitive Measurement of Glomerular Permeability in Mice with Fluorescein Isothiocyanate-polysucrose 70
09:16

Highly Sensitive Measurement of Glomerular Permeability in Mice with Fluorescein Isothiocyanate-polysucrose 70

Published on: August 9, 2019

7.2K
Assessment of Kidney Function in Mouse Models of Glomerular Disease
09:16

Assessment of Kidney Function in Mouse Models of Glomerular Disease

Published on: June 30, 2018

18.3K
Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins
09:12

Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins

Published on: January 18, 2019

9.8K

Area of Science:

  • Nephrology
  • Molecular Biology
  • Cell Biology

Background:

  • The glomerular basement membrane (GBM) is crucial for kidney filtration, composed mainly of laminins, type IV collagen, nidogens, and proteoglycans.
  • It also contains growth factors and undergoes significant changes during kidney development.
  • Dysfunction of the GBM is implicated in hereditary diseases (e.g., Alport syndrome) and acquired conditions like diabetes.

Purpose of the Study:

  • To review the current understanding of glomerular basement membrane (GBM) composition, assembly, and role in kidney health and disease.
  • To highlight advancements in studying basement membrane structure and function.
  • To discuss the implications of GBM defects in hereditary and acquired kidney diseases and explore potential therapeutic strategies.

Main Methods:

  • Review of recent literature on GBM structure, composition, and disease associations.
  • Analysis of new technologies for studying basement membrane assembly.
  • Synthesis of information on genetic defects and acquired conditions affecting the GBM.

Main Results:

  • The GBM is a complex macromolecular structure essential for glomerular filtration.
  • Defects in GBM components lead to hereditary kidney diseases like Alport and Pierson syndromes.
  • Acquired diseases, including diabetes mellitus, also impact GBM integrity.

Conclusions:

  • Understanding GBM composition and assembly is critical for diagnosing and treating kidney diseases.
  • New therapeutic approaches targeting GBM repair or component replacement hold promise for future treatments.
  • Continued research into GBM maintenance and turnover is essential for advancing patient care.