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Related Concept Videos

Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

686
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
686
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

854
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
854
Electrophoresis: Overview01:20

Electrophoresis: Overview

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Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
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Controlled-Current Coulometry: Overview01:27

Controlled-Current Coulometry: Overview

521
Controlled current coulometry, also known as amperostatic coulometry, is a technique used in electrochemical analysis to measure the quantity of a substance through the controlled passage of current. It involves the application of a constant current to an electrochemical cell containing the analyte of interest. As the current flows through the cell, the analyte undergoes a redox reaction at the electrode surface, resulting in a charge transfer. By monitoring the time required for a certain...
521
Controlled-Potential Coulometry: Electrolytic Methods01:17

Controlled-Potential Coulometry: Electrolytic Methods

513
Controlled-potential coulometry, also known as potentiostatic coulometry, employs a three-electrode system in which the working electrode's potential is precisely regulated using a potentiostat. Platinum working electrodes are utilized for positive potentials, while mercury pool electrodes are favored for extremely negative potentials. The platinum counter electrode is separated from the analyte using a membrane or salt bridge to avoid interference in the analysis.
The chosen potential...
513
Controlled-Current Coulometry: Coulometric Titration01:18

Controlled-Current Coulometry: Coulometric Titration

396
Coulometric titrations are a form of titrimetric analysis where the reagent is generated electrically, and its amount is evaluated based on current and generating time. The electron serves as the standard reagent. The procedure is similar to conventional titrations, such as endpoint detection.
The fundamental requirements for coulometric titrations are (1) 100% efficiency in the reagent-generating electrode reaction and (2) a stoichiometric and preferably rapid reaction between the generated...
396

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Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
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Voltage control for microchip capillary electrophoresis analyses.

Rick Bosma1, Jasen Devasagayam1, Rahul Eswar1

  • 1School of Engineering, College of Engineering and Physical Sciences, University of Guelph, Guelph, ON, Canada.

Electrophoresis
|August 26, 2020
PubMed
Summary
This summary is machine-generated.

A new, low-cost voltage sequencer instrument enables microchip capillary electrophoresis (MCE) actuation. This affordable device simplifies MCE experiments, making them accessible for industries like dairy testing.

Keywords:
Automated voltage sequencerHigh voltageLab-on-a-chipMicrochip capillary electrophoresis

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Area of Science:

  • Analytical Chemistry
  • Instrumentation Science

Background:

  • Microchip capillary electrophoresis (MCE) requires precise voltage control for actuation.
  • Existing MCE instrumentation can be expensive and complex to implement.
  • Accurate detection of contaminants like antibiotics in food products is crucial for industry and public health.

Purpose of the Study:

  • To develop and present an inexpensive and user-friendly voltage sequencer instrument for MCE.
  • To demonstrate the instrument's capability in detecting ciprofloxacin in milk samples using MCE.

Main Methods:

  • Designed and assembled a four-channel voltage sequencer using a microcontroller and peripheral interface controller.
  • Integrated the voltage sequencer with a fluorescence spectroscopy MCE sensor.
  • Utilized pinched sample injection and electrophoretic separation for ciprofloxacin detection.

Main Results:

  • The voltage sequencer is constructed for under 60 USD and measures 105 × 143 × 45 mm.
  • The instrument successfully generates proportional voltage signals for MCE actuation.
  • Demonstrated effective detection of ciprofloxacin in milk samples, validating its performance.

Conclusions:

  • The developed voltage sequencer is a cost-effective and accessible tool for MCE applications.
  • This instrumentation facilitates MCE experiments, particularly for quality control in the dairy industry.
  • The low-cost nature of the device promotes wider adoption of MCE technology in industrial settings.