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Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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Updated: Dec 11, 2025

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells

Published on: May 28, 2021

Unveiling Cells' Local Environment during Cryopreservation by Correlative In Situ Spatial and Thermal Analyses.

Kankan Qin¹, Corentin Eschenbrenner¹, Felix Ginot²

¹Sorbonne Université, UMR 7574, Laboratoire de Chimie de la Matière Condensée de Paris, F-75005 Paris, France.

The Journal of Physical Chemistry Letters

|August 26, 2020

Summary

Cryopreservation extends cell and tissue lifespan, but the freezing process remains unclear. This study visualizes the cellular environment during freezing, identifying key interactions that define cell survival during cryopreservation.

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Last Updated: Dec 11, 2025

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A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography

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Published on: March 15, 2021

Area of Science:

Cell Biology
Biophysics
Materials Science

Background:

Cryopreservation is crucial for preserving cells and tissues in biology and medicine.
The precise physicochemical environment cells experience during freezing is not fully understood.
Understanding this environment is key to improving cryopreservation techniques.

Purpose of the Study:

To elucidate the cellular physicochemical environment during cryopreservation.
To develop a method for visualizing and quantifying the cell's local environment during freezing.
To identify critical events influencing cell survival during the cryopreservation process.

Main Methods:

Coupling *in situ* microscopic directional freezing with freezing-medium phase diagrams.
Visualizing cells and their surroundings during the freezing process.
Extracting the spatial distribution of the freezing medium around cells.

Main Results:

Developed a tool to describe the cell vicinity at any freezing point.
Identified two major events defining the cellular local environment: interaction with the ice front and the newly introduced vitreous moving front.
Provided insights into the physicochemical cues cells encounter during cryopreservation.

Conclusions:

The correlative strategy offers a new way to study the cell's local environment during freezing.
This approach can be applied to clinically relevant cells.
Findings may guide the development of improved cryoprotective media based on local physicochemical cues.