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Long non-coding RNA PICSAR knockdown inhibits the progression of cutaneous squamous cell carcinoma by regulating

Xiaoyan Lu1, Quan Gan1, Caibin Gan1

  • 1Department of Dermatology, Xinxiang Central Hospital, Xinxiang, Henan, China.

Life Sciences
|August 26, 2020
PubMed
Abstract

Insights

Knocking down p38 inhibiting cutaneous squamous cell carcinoma associated lincRNA (PICSAR) in cutaneous squamous cell carcinoma (CSCC) inhibits cancer progression. PICSAR regulates the miR-125b/YAP1 axis, offering a potential therapeutic target for CSCC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Cutaneous squamous cell carcinoma (CSCC) is a prevalent skin cancer.
  • Long non-coding RNAs (lncRNAs) play critical roles in cancer development.
  • The specific function of PICSAR in CSCC remains largely unknown.

Purpose of the Study:

  • To elucidate the role and mechanism of p38 inhibiting cutaneous squamous cell carcinoma associated lincRNA (PICSAR) in CSCC.
  • To investigate the regulatory pathway involving PICSAR, miR-125b, and YAP1 in CSCC.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) to measure PICSAR, miR-125b, and YAP1 expression.
  • Cell proliferation (CCK-8), apoptosis (flow cytometry), and invasion (Transwell) assays.
  • Bioinformatics, dual-luciferase reporter, and RNA immunoprecipitation (RIP) assays to confirm molecular interactions.
  • Western blot for YAP1 protein levels and a mice xenograft model for in vivo validation.

Main Results:

  • PICSAR expression was significantly upregulated in CSCC tissues and cells.
  • PICSAR knockdown inhibited CSCC cell proliferation and invasion while promoting apoptosis.
  • PICSAR directly interacted with miR-125b, and miR-125b targeted YAP1.
  • PICSAR positively regulated YAP1, while miR-125b negatively regulated it.
  • Interference with PICSAR suppressed tumor growth by modulating the miR-125b/YAP1 axis.

Conclusions:

  • PICSAR knockdown exerts anti-cancer effects in CSCC by regulating the miR-125b/YAP1 axis.
  • The PICSAR/miR-125b/YAP1 pathway represents a novel therapeutic target for CSCC treatment.
Keywords:
Cutaneous squamous cell carcinomaPICSARYAP1miR-125b

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