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Curcumin and Quercetin-Loaded Nanoemulsions: Physicochemical Compatibility Study and Validation of a Simultaneous
Gustavo Richter Vaz1,2, Adryana Clementino3, Juliana Bidone4
1Laboratório de Nanotecnologia Aplicada à Saúde, Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal do Rio Grande, Rio Grande 96210-900 (RS), Brazil.
Nanomaterials (Basel, Switzerland)
|August 27, 2020
Summary
This study developed a novel HPLC-UV/Vis method for simultaneously quantifying curcumin (CUR) and quercetin (QU) in nanoemulsions. The validated method ensures high encapsulation efficiency and stability for intranasal drug delivery systems.
Area of Science:
- Pharmaceutical Sciences
- Nanotechnology
- Analytical Chemistry
Background:
- Biphasic oil/water nanoemulsions are promising for intranasal delivery of curcumin (CUR) and quercetin (QU).
- These systems offer high drug entrapment, simultaneous administration, and protection against degradation.
- Understanding physicochemical and biological performance is crucial for nanomedicine development.
Purpose of the Study:
- To develop and validate a new HPLC-UV/Vis method for simultaneous determination of CUR and QU in nanoemulsions.
- To assess the stability of CUR and QU within the nanoemulsion formulation.
- To determine encapsulation efficiencies and stability in simulated biological fluids.
Main Methods:
- Stability testing using X-ray powder diffraction, differential scanning calorimetry (DSC), and thermogravimetric analyses (TGA).
- Development and validation of a selective, linear, precise, robust, and accurate HPLC-UV/Vis method.
- Application of the method for quantifying encapsulation efficiency and stability in biological fluids.
Main Results:
- X-ray diffraction and thermal analyses confirmed no significant solid-state interactions between CUR and QU.
- CUR and QU demonstrated stability within the nanoemulsion production temperature range.
- The validated HPLC-UV/Vis method exhibited excellent linearity (R² > 0.9997), precision (RSD < 3%), and accuracy (100 ± 5% recovery).
- High encapsulation efficiencies (>99%) were achieved for both CUR and QU in the nanoemulsions.
- The method demonstrated low limits of detection and quantification, suitable for drug release studies.
Conclusions:
- A novel, validated HPLC-UV/Vis method enables simultaneous quantification of CUR and QU in nanoemulsions.
- The nanoemulsion formulation is stable and effectively encapsulates CUR and QU for intranasal delivery.
- This method is essential for the development and quality control of CUR/QU co-loaded nanoemulsions.
