Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulated Protein Degradation02:58

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA molecules by RNA...
Regulated Protein Degradation02:58

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA molecules by RNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of a genetically attenuated Cryptosporidium parvum strain in the calf clinical model of cryptosporidiosis.

The Journal of infectious diseases·2026
Same author

Motor, not cognitive, performance relates to amyloid status in normal older adults.

Alzheimer's & dementia (Amsterdam, Netherlands)·2026
Same author

Multi-modal machine learning for predicting amyloid positivity using on-ramp driving.

Alzheimer's & dementia (Amsterdam, Netherlands)·2025
Same author

Evaluating the factor structure and construct validity of the NIH toolbox in older adults, with a focus on cognitive normalcy and amnestic mild cognitive impairment: considerations for diversity, including insights from persons over 85 years of age and Black older Americans.

Journal of the International Neuropsychological Society : JINS·2024
Same author

The Cryptosporidium signaling kinase CDPK5 plays an important role in male gametogenesis and parasite virulence.

Cell reports·2024
Same author

Tyrosine Kinase Inhibitors Display Potent Activity against Cryptosporidium parvum.

Microbiology spectrum·2022

Related Experiment Video

Updated: Jul 3, 2026

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
14:06

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

46.8K

A Conditional Protein Degradation System To Study Essential Gene Function in Cryptosporidium parvum.

Hadi H Choudhary1, Maria G Nava1, Brina E Gartlan1

  • 1Department of Pathobiology, College of Veterinary Medicine, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.

Mbio
|August 27, 2020
PubMed
Summary
This summary is machine-generated.

Researchers developed a new conditional genetic system for Cryptosporidium parvum, enabling the study of essential genes like CDPK1. This tool is crucial for developing new treatments against cryptosporidiosis, a major cause of childhood diarrhea.

Keywords:
CRISPR/Cas9Cryptosporidiumapicomplexan parasiteconditional systemdrug targetsmolecular genetics

More Related Videos

Reporter-based Growth Assay for Systematic Analysis of Protein Degradation
07:47

Reporter-based Growth Assay for Systematic Analysis of Protein Degradation

Published on: November 6, 2014

11.0K
Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae
10:57

Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae

Published on: February 16, 2015

10.0K

Related Experiment Videos

Last Updated: Jul 3, 2026

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
14:06

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

46.8K
Reporter-based Growth Assay for Systematic Analysis of Protein Degradation
07:47

Reporter-based Growth Assay for Systematic Analysis of Protein Degradation

Published on: November 6, 2014

11.0K
Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae
10:57

Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae

Published on: February 16, 2015

10.0K

Area of Science:

  • Molecular Parasitology
  • Genetics
  • Drug Discovery

Background:

  • Cryptosporidium spp. are significant causes of diarrheal disease, particularly in young children and immunocompromised individuals.
  • Existing treatments for cryptosporidiosis are limited, and no vaccines are available, highlighting the need for novel therapeutic targets.
  • Studying essential genes in Cryptosporidium is vital for developing new drugs, but current genetic tools are insufficient for essential gene analysis.

Purpose of the Study:

  • To develop the first conditional genetic system for Cryptosporidium parvum to enable the study of essential gene functions.
  • To validate the system using calcium-dependent protein kinase-1 (CDPK1), a known drug target, to assess its essentiality and role in parasite proliferation.

Main Methods:

  • Development of a conditional system using the Escherichia coli dihydrofolate reductase degradation domain (DDD) and trimethoprim (TMP).
  • Generation of transgenic C. parvum expressing DDD-tagged CDPK1.
  • Utilized CRISPR/Cas9 for gene editing and epitope tagging of CDPK1.
  • Assessed TMP-mediated regulation of CDPK1 levels and parasite sensitivity to kinase inhibitors.

Main Results:

  • Established that cdpk1 is essential for C. parvum survival, as it is refractory to gene deletion.
  • Demonstrated TMP-mediated conditional knockdown of CDPK1 levels in transgenic parasites.
  • Showed that conditional CDPK1 knockdown impairs parasite proliferation and increases sensitivity to kinase inhibitors.

Conclusions:

  • The developed conditional system provides a powerful new tool for studying essential genes in Cryptosporidium spp.
  • This system facilitates a deeper understanding of C. parvum biology, crucial for accelerating the development of new anticryptosporidial therapies.
  • The findings underscore the utility of conditional gene manipulation for identifying and validating drug targets in parasitic protozoa.