Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Tumor Microenvironment02:17

The Tumor Microenvironment

7.5K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
7.5K
Metastasis02:30

Metastasis

6.2K
Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
6.2K
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

3.3K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
3.3K
Tumor Progression02:07

Tumor Progression

7.1K
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
7.1K
Drugs that Stabilize Microtubules01:15

Drugs that Stabilize Microtubules

2.5K
Microtubules are dynamic structures that undergo cycles of catastrophe and rescue. The microtubules play a central role in cell division by forming the spindle apparatus for segregating the chromosomes. This makes them ideal targets for regulating dividing cells in tumors and malignant cancer cells. Microtubule stabilizing drugs help stabilize the microtubule formation and promote its polymerization. Paclitaxel was the first microtubule stabilizing agent used as anticancer drug in chemotherapy...
2.5K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

3.2K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lipogenesis-driven EGFR palmitoylation enables metastatic immune evasion in triple-negative breast cancer.

bioRxiv : the preprint server for biology·2026
Same author

Assessment of Functional and Radiological Outcomes of Multi-Lock Retrograde Nailing in Lower End of Femur.

Indian journal of orthopaedics·2026
Same author

Tumor immune microenvironment reconstitution in patient-derived organoids enables therapy modeling for NSCLC.

Cell reports methods·2026
Same author

Impact of End-Stage Renal Disease on Outcomes of Rotational Atherectomy-Assisted Percutaneous Coronary Interventions: Insights From a National Inpatient Cohort.

Catheterization and cardiovascular interventions : official journal of the Society for Cardiac Angiography & Interventions·2026
Same author

Predictors of ischemic and hemorrhagic strokes among adults undergoing left ventricular assist device implantation at urban teaching centers in the United States.

Archives of medical sciences. Atherosclerotic diseases·2026
Same author

Longitudinal Genomic Analysis of Five Cases of Recurrent Thymomas.

Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer·2025
Same journal

Peptidomics in the Spotlight: Advanced Sample Treatment Techniques and Analytical Insights.

Advances in experimental medicine and biology·2026
Same journal

Methods for the Investigation of Protein-Ligands Interactions.

Advances in experimental medicine and biology·2026
Same journal

Sample Preparation Strategies for Microbial Cell Surface Proteomics: Integrating Shaving and Shotgun Approaches.

Advances in experimental medicine and biology·2026
Same journal

Proteomic Sample Preparation for the Petroleum Industry: A Biocorrosion Case Study.

Advances in experimental medicine and biology·2026
Same journal

Proteomic and Functional Comparison of Extracellular Vesicles from Wild-Type and Lyn-Deficient Stromal Cells.

Advances in experimental medicine and biology·2026
Same journal

Proteomic Analysis of Histone Sequence Variants and Post-translationally Modified Forms.

Advances in experimental medicine and biology·2026
See all related articles

Related Experiment Video

Updated: Dec 10, 2025

Isolation of Proximal Fluids to Investigate the Tumor Microenvironment of Pancreatic Adenocarcinoma
05:44

Isolation of Proximal Fluids to Investigate the Tumor Microenvironment of Pancreatic Adenocarcinoma

Published on: November 5, 2020

4.8K

Thrombospondin in Tumor Microenvironment.

Divya Ramchandani1, Vivek Mittal2

  • 1Department of Cardiothoracic Surgery, Weill Cornell Medicine, New York, NY, USA.

Advances in Experimental Medicine and Biology
|August 27, 2020
PubMed
Summary
This summary is machine-generated.

Thrombospondins (TSPs) are extracellular matrix proteins with dual roles in cancer, promoting and suppressing tumors. TSP-1, a key TSP, inhibits T cells and Tregs, impacting the tumor microenvironment and offering therapeutic targets.

Keywords:
AdhesionAngiogenesisCD36CD47CancerDormancyImmune systemInvasionMetastasisMicroenvironmentMigrationPre-metastatic nicheSpreadingTGF-βThrombospondin

More Related Videos

Three-dimensional Cell Culture Model for Measuring the Effects of Interstitial Fluid Flow on Tumor Cell Invasion
07:41

Three-dimensional Cell Culture Model for Measuring the Effects of Interstitial Fluid Flow on Tumor Cell Invasion

Published on: July 25, 2012

17.0K
A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery
07:20

A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery

Published on: February 28, 2020

10.3K

Related Experiment Videos

Last Updated: Dec 10, 2025

Isolation of Proximal Fluids to Investigate the Tumor Microenvironment of Pancreatic Adenocarcinoma
05:44

Isolation of Proximal Fluids to Investigate the Tumor Microenvironment of Pancreatic Adenocarcinoma

Published on: November 5, 2020

4.8K
Three-dimensional Cell Culture Model for Measuring the Effects of Interstitial Fluid Flow on Tumor Cell Invasion
07:41

Three-dimensional Cell Culture Model for Measuring the Effects of Interstitial Fluid Flow on Tumor Cell Invasion

Published on: July 25, 2012

17.0K
A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery
07:20

A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery

Published on: February 28, 2020

10.3K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Oncology

Background:

  • Thrombospondins (TSPs) are extracellular matrix proteins with diverse roles in physiological and pathological processes.
  • TSPs exhibit context-dependent oncogenic and tumor-suppressive functions due to multiple receptor-binding domains.
  • TSPs are crucial components of the tumor microenvironment, influencing angiogenesis, cell migration, and immune responses.

Purpose of the Study:

  • To explore the multifaceted roles of Thrombospondins (TSPs) within the tumor microenvironment.
  • To discuss the structural domains and functional implications of TSPs in cancer progression and metastasis.
  • To review anti-metastatic therapeutic strategies targeting TSPs.

Main Methods:

  • Literature review of Thrombospondin (TSP) functions in cancer.
  • Analysis of TSP structural domains and their relation to biological activity.
  • Examination of therapeutic approaches utilizing TSP-mimetic peptides and antibody blockade.

Main Results:

  • TSPs possess both tumor-promoting and tumor-suppressive qualities, acting as key regulators in the tumor microenvironment.
  • TSP-1 demonstrates antiangiogenic capacity and mediates immunosuppression by inhibiting T cells and inducing regulatory T cells (Tregs).
  • A potential feedback loop between TGF-β and TSP-1 in tumor growth and inflammation was identified.

Conclusions:

  • Thrombospondins (TSPs) play complex roles in cancer, influencing cell adhesion, migration, angiogenesis, and immune evasion.
  • TSP-1's immunosuppressive functions and antiangiogenic properties make it a significant target for anti-cancer therapies.
  • Targeting TSPs through therapeutic strategies like peptide mimetics or antibody blockade shows promise for anti-metastatic approaches.