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A gas chromatographic/mass spectrometric assay for catechol estrogens in microsomal incubations: comparison with a
1Department of Medicinal Chemistry, School of Pharmacy, University of Washington, Seattle 98195.
Biomedical & Environmental Mass Spectrometry
|February 1, 1988
Abstract:
A gas chromatographic/mass spectrometric assay for quantifying two catechol estrogens, 2-hydroxyestradiol and 4-hydroxyestradiol, in microsomal preparations is described. The assay employs deuterium-labeled analogs of the catechol estrogens as internal standards and permits quantification of catechol estrogens, in microsomal incubations, at low (1-2) microM concentrations. The compounds are analyzed as their trimethylsilyl derivatives following separation by capillary gas chromatography.