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Updated: May 11, 2026

In Vitro Disassembly of Influenza A Virus Capsids by Gradient Centrifugation
Published on: March 27, 2016
Capsule loss in H. influenzae type b occurs by recombination-mediated disruption of a gene essential for
Abstract:
The capsulation locus cap in H. influenzae type b contains directly repeated segments of DNA flanking a bridge region. Here we show that this bridge region contains a gene, bexA, encoding a 24.7 kd protein essential for export of capsular polysaccharide. bexA is disrupted, with loss of part of its coding sequence, in the spontaneous reduction of the duplicated cap locus to single-copy that accompanies loss of capsule expression. The predicted amino acid sequence of BexA aligns significantly with that of MalK from E. coli and with HisP and OppD of S. typhimurium. Thus, polysaccharide export might occur via an energy-dependent transporter with similarities to those identified for the import of various substrates into Gram-negative bacteria, BexA being the "energizer" of the transporter.
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