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Updated: Dec 10, 2025
10:17
Analysis of Schwann-astrocyte Interactions Using In Vitro Assays
Published on: January 13, 2011
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FGF5 Regulates Schwann Cell Migration and Adhesion.
Bing Chen1, Rong Hu2, Qing Min3
1Department of Neurology, The Affiliated Huai'an No.1 People's Hospital of Nanjing Medical University, Huai'an, China.
Frontiers in Cellular Neuroscience
|August 28, 2020
Summary
Fibroblast growth factor 5 (FGF5) is upregulated in peripheral nerve injury. FGF5 promotes Schwann cell migration and adhesion, suggesting an autocrine role in nerve repair.
Area of Science:
- Neuroscience
- Cell Biology
- Regenerative Medicine
Background:
- Fibroblast growth factors (FGFs) are crucial for tissue repair.
- FGF5 is upregulated in Schwann cells after peripheral nerve injury.
- Its specific role in nerve regeneration remains unclear.
Purpose of the Study:
- To investigate FGF5 expression and its receptors in injured sciatic nerves.
- To determine the functional effects of FGF5 on primary Schwann cells.
Main Methods:
- Microarray and mRNA sequencing
- RT-PCR and qPCR
- Western blotting and immunostaining
- Primary rat Schwann cell culture and treatment
Main Results:
- FGF5 is highly upregulated in mouse distal sciatic nerve Schwann cells post-injury.
- FGFR1 and FGFR2 are expressed in Schwann cells before and after injury.
- FGF5 inhibits ERK1/2 MAP kinase activity.
- FGF5 promotes Schwann cell migration and adhesion via N-cadherin upregulation.
Conclusions:
- FGF5 is an autocrine regulator of Schwann cell migration and adhesion.
- FGF5 plays a significant role in peripheral nerve regeneration.
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