Related Experiment Video
Updated: Dec 10, 2025
11:44
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
Published on: March 30, 2019
7.9K
circAFF1 Aggravates Vascular Endothelial Cell Dysfunction Mediated by miR-516b/SAV1/YAP1 Axis
Hong-Guang Wang1,2,3,4, Hua Yan2,3, Chen Wang3,5
1College of Pharmacy, Nankai University, Tianjin, China.
Frontiers in Physiology
|August 28, 2020
Summary
Hypoxia-induced circular RNA AFF1 (circAFF1) activates SAV1/YAP1, causing vascular endothelial dysfunction. This circAFF1/miR-516b/SAV1/YAP1 pathway may serve as an early diagnostic marker for vascular diseases.
Area of Science:
- Molecular Biology
- Vascular Biology
- Biochemistry
Background:
- Hypoxia-induced vascular endothelial damage underlies many vascular diseases.
- The role of circular RNAs in hypoxic vascular injury remains largely unknown.
Purpose of the Study:
- To investigate the role of circular RNA AFF1 (circAFF1) in hypoxic vascular injury.
- To elucidate the underlying molecular mechanism involving the SAV1/YAP1 pathway.
- To assess the potential diagnostic value of circAFF1 in related diseases.
Main Methods:
- Induction of hypoxia in HUV-EC-C and HBEC-5i cells using CoCl2.
- Quantitative analysis of circAFF1 expression.
- Assessment of cell proliferation, tube formation, and migration.
- Investigation of the circAFF1/miR-516b/SAV1/YAP1 interaction.
Main Results:
- circAFF1 was significantly upregulated under hypoxic conditions.
- Abnormal circAFF1 expression inhibited vascular endothelial cell proliferation, tube formation, and migration.
- circAFF1 acts by sequestering miR-516b, leading to SAV1 release and YAP1 phosphorylation.
- circAFF1 was found to be upregulated in patients with subarachnoid hemorrhage.
Conclusions:
- circAFF1 plays a critical role in hypoxia-induced vascular endothelial dysfunction.
- The circAFF1/miR-516b/SAV1/YAP1 axis is a key pathway in this process.
- circAFF1 demonstrates potential as an early diagnostic biomarker for hypoxia-related vascular diseases.
More Related Videos
Related Concept Videos
01:24Regulation of Angiogenesis and Blood Supply
3.2K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.2K
01:10Mechanism of Angiogenesis
6.4K
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
6.4K
01:16TGF - β Signaling Pathway
9.9K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
9.9K
01:07MAPK Signaling Cascades
7.5K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
7.5K
01:17Intracellular Signaling Affects Focal Adhesions
3.3K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
3.3K
03:14Notch Signaling Pathway
6.0K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
6.0K