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Advanced Microscopy Reveals Complex Developmental and Subcellular Localization Patterns of ANNEXIN 1 in Arabidopsis.

Michaela Tichá1, Hendrik Richter2, Miroslav Ovečka1

  • 1Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University Olomouc, Olomouc, Czechia.

Frontiers in Plant Science
|August 28, 2020
PubMed
Summary
This summary is machine-generated.

Annexin 1 (ANN1) shows complex developmental and subcellular localization in Arabidopsis, accumulating at root tips and plasma membranes. This study reveals ANN1

Keywords:
Arabidopsis thalianaannexin 1cell divisiondevelopmentlattice light-sheet fluorescence microscopylight-sheet fluorescence microscopyplasma membranesubcellular localization

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Area of Science:

  • Plant cell biology
  • Molecular plant science
  • Cellular and subcellular localization

Background:

  • Annexins are conserved protein families involved in plant cell processes.
  • Annexin 1 (ANN1) is abundant in Arabidopsis, particularly in roots.
  • Subcellular localization of ANN1 during development was previously uncharacterized.

Purpose of the Study:

  • To determine the developmental and subcellular localization of ANN1 in living Arabidopsis plants.
  • To investigate the dynamic changes in ANN1 localization during plant development.
  • To explore potential roles of ANN1 in cellular processes like growth and stress response.

Main Methods:

  • Utilized advanced light-sheet fluorescence microscopy (LSFM) for long-term imaging of GFP-tagged ANN1 in Arabidopsis.
  • Applied immunolocalization techniques to confirm subcellular associations.
  • Observed ANN1-GFP localization in various post-embryonic organs, including roots and aerial tissues.

Main Results:

  • ANN1-GFP accumulated in root caps, elongating trichoblasts, and root hair tips, but was depleted in meristematic dividing cells.
  • Subcellularly, ANN1-GFP localized to the plasma membrane, vesicles, and mitotic/cytokinetic microtubular arrays.
  • Relocation to the plasma membrane and Hechtian strands during plasmolysis suggests an osmoprotective role.

Conclusions:

  • ANN1 exhibits complex, dynamic localization patterns throughout Arabidopsis development.
  • ANN1 is associated with cell growth, division, and potentially stress responses.
  • LSFM provides unprecedented insights into ANN1 subcellular dynamics in living plants.