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Changes in peripheral blood mononuclear cells' mRNA expression of TLRs and CD14 during puerperal metritis in dairy
R Asadpour1, A Farhadi2, R Jafari Jozani1
1Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
Background:
Peripheral blood mononuclear cells (PBMCs), commonly referred to as lymphocytes and monocytes, representing cells of the innate and adaptive immune systems.
Aims:
To find out whether changes in PBMCs' mRNA expression of pattern recognition receptors (PRRs) are associated with puerperal metritis in Holstein cows.
Methods:
Peripheral blood mononuclear cells were collected from 20 cows with puerperal metritis and 20 cows without metritis at 10 days postpartum. Expression of toll-like receptors 2 and 4 (TLR2 and TLR4), and cluster of differentiation 14 (CD14) genes were assessed in PBMCs using a quantitative real time-polymerase chain reaction (qRT-PCR) technique. The data was normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a reference gene, and 2-∆∆Ct methodology was used for relative quantification.
Results:
The results of the present study demonstrated that the expression of TLR4 (P=0.04) and CD14 (P=0.008) was significantly greater in cows with puerperal metritis compared to the control group. However, the expression of TLR2 (P=0.06) was not significantly different between cows with puerperal metritis and healthy cows.
Conclusion:
This study suggests that puerperal metritis significantly increases the expression of TLR4 and CD14 genes in the PBMCs which contributes to the proper stimulation of inflammation and uterine clearance of bacteria soon after calving.
Insights
Puerperal metritis in Holstein cows is linked to increased expression of Toll-like receptor 4 (TLR4) and CD14 genes in peripheral blood mononuclear cells (PBMCs). This suggests a role in the cow's inflammatory response post-calving.
Area of Science:
- Veterinary Immunology
- Bovine Infectious Diseases
- Molecular Diagnostics
Background:
- Peripheral blood mononuclear cells (PBMCs) are key components of innate and adaptive immunity.
- Puerperal metritis is a common postpartum uterine disease in dairy cows.
- Pattern recognition receptors (PRRs) play a crucial role in immune responses to pathogens.
Purpose of the Study:
- To investigate the association between puerperal metritis and mRNA expression of specific PRRs in Holstein cows.
- To determine if Toll-like receptor 2 (TLR2), Toll-like receptor 4 (TLR4), and cluster of differentiation 14 (CD14) gene expression in PBMCs differs between healthy and metritic cows.
Main Methods:
- Peripheral blood mononuclear cells (PBMCs) were collected from Holstein cows with and without puerperal metritis at 10 days postpartum.
- Quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure the mRNA expression of TLR2, TLR4, and CD14.
- Gene expression data was normalized to GAPDH and analyzed using the 2-∆∆Ct method.
Main Results:
- Significantly higher expression of TLR4 (P=0.04) and CD14 (P=0.008) was observed in PBMCs from cows with puerperal metritis compared to healthy controls.
- No significant difference in TLR2 expression was found between the groups (P=0.06).
Conclusions:
- Puerperal metritis is associated with elevated expression of TLR4 and CD14 genes in bovine PBMCs.
- Increased TLR4 and CD14 expression likely contributes to the inflammatory response and bacterial clearance in the uterus post-calving.
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