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Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages
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Modulation of Macrophage Function by Lactobacillus-Conditioned Medium.

Yashaswini Seenappanahalli Nanjundaiah1, David A Wright1, Anwar R Baydoun2

  • 1School of Health and Life Sciences, Teesside University, Middlesbrough, United Kingdom.

Frontiers in Cell and Developmental Biology
|August 28, 2020
PubMed
Summary
This summary is machine-generated.

Probiotic Lactobacillus rhamnosus GG conditioned media enhanced macrophage bacterial killing by 6-fold. This process involved increased free radical production, suggesting a mechanism for modulating host inflammation.

Keywords:
Lactobacillus rhamnosus GGfree radicalsmacrophagenitric oxidephagocytosis

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Area of Science:

  • Immunology
  • Microbiology
  • Gastroenterology

Background:

  • Probiotics are microbial supplements with potential immunomodulatory effects.
  • The precise mechanisms behind probiotic-mediated immune responses remain unclear.
  • Investigating probiotic effects on macrophage function is crucial for understanding their health benefits.

Purpose of the Study:

  • To investigate the impact of Lactobacillus rhamnosus GG conditioned media (LGG-CM) on macrophage phagocytosis and killing of Escherichia coli.
  • To elucidate the role of free radicals and nitric oxide (NO) in LGG-CM-mediated bacterial clearance.

Main Methods:

  • Utilized the gentamicin protection assay to quantify bacterial killing by macrophages.
  • Co-incubated macrophages with Escherichia coli and monitored bacterial survival over time.
  • Measured free radical production via NADPH oxidase activation and nitric oxide generation.

Main Results:

  • LGG-CM significantly enhanced the killing of Escherichia coli by macrophages approximately 6-fold compared to controls.
  • Enhanced bacterial killing was associated with increased free radical production, primarily through NADPH oxidase activation.
  • Nitric oxide generation was minimally affected by LGG-CM.

Conclusions:

  • LGG-CM enhances macrophage-mediated bacterial clearance, potentially by increasing oxidative stress.
  • This probiotic-derived factor may help control microbial load within macrophages.
  • Modulation of inflammatory signals by LGG-CM could offer protective benefits against host tissue damage.