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Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
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Genetic sensor-regulators functional in Clostridia.

SongI Han1, Younghwan Kim2, Mukund Karanjikar2

  • 1Department of Bioengineering, Rice University, Houston, TX, 77005, USA.

Journal of Industrial Microbiology & Biotechnology
|August 28, 2020
PubMed
Summary
This summary is machine-generated.

Genetic circuits from Clostridium acetobutylicum were functional in Clostridium species, enabling reporter protein expression. A xylose-inducible promoter demonstrated titratable control, useful for woody hydrolysate applications.

Keywords:
Beta-galactosidaseClostridium acetobutylicumRegulatorSensormCherryOpt

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Area of Science:

  • Synthetic biology
  • Microbial biotechnology
  • Genetic engineering

Background:

  • Clostridium species are crucial for industrial fermentations.
  • Understanding and engineering their genetic regulatory elements is key for optimizing bioprocesses.
  • Natural regulatory elements offer potential for controlled gene expression.

Purpose of the Study:

  • To assess the functionality of natural genetic circuits from Clostridium acetobutylicum in its native host.
  • To evaluate the transferability and performance of these circuits in other Clostridium species.
  • To characterize inducible promoters for controlled gene expression.

Main Methods:

  • Construction and testing of genetic circuits with reporter genes (mCherryOpt, beta-galactosidase).
  • Characterization of promoters and regulators involved in carbon source utilization.
  • Assessing gene expression in response to specific inducers and carbon source mixtures.

Main Results:

  • Most tested genetic circuits were functional in Clostridium acetobutylicum ATCC 824 upon inducer addition.
  • These genetic circuits demonstrated successful transfer and function in Clostridium beijerinckii NCIMB 8052.
  • A xylose-inducible promoter provided titratable and robust reporter protein expression.
  • Expression levels correlated with the presence of specific carbohydrates, including in woody hydrolysate.

Conclusions:

  • Natural regulatory elements of Clostridium acetobutylicum can be effectively utilized in heterologous hosts.
  • Inducible genetic circuits offer precise control over gene expression in Clostridium species.
  • The characterized xylose-inducible system is suitable for applications involving complex carbon sources like woody hydrolysates.