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Quantitation of a transformation-sensitive, adhesive cell surface glycoprotein. Decrease of several untransformed

K M Yamada, S S Yamada, I Pastan

    The Journal of Cell Biology
    |August 1, 1977
    PubMed
    Summary
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    Researchers quantified the large external transformation-sensitive (LETS) glycoprotein in cells. A significant decrease in LETS glycoprotein was observed in continuous cell lines, independent of cellular transformation.

    Area of Science:

    • Cell Biology
    • Biochemistry
    • Oncology

    Background:

    • The large external transformation-sensitive (LETS) glycoprotein is a cell surface protein implicated in cellular morphology and behavior.
    • Transformation-sensitive proteins are often downregulated in continuous cell lines and cancer cells.

    Purpose of the Study:

    • To quantify the levels of the LETS glycoprotein in various untransformed and continuous cell lines.
    • To investigate the relationship between LETS glycoprotein expression and cellular transformation.

    Main Methods:

    • Quantification of LETS glycoprotein using SDS-polyacrylamide gel electrophoresis.
    • Analysis of [14C]glucosamine labeling and immunofluorescent staining to assess glycoprotein levels and distribution.
    • Comparison of LETS glycoprotein expression in early passage cells versus continuous cell lines.

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    Main Results:

    • LETS glycoprotein constituted 1-3% of total cellular protein in early passage cell types.
    • In contrast, LETS glycoprotein comprised less than 0.15% of total protein in four of six continuous cell lines.
    • Decreased LETS glycoprotein levels correlated with altered [14C]glucosamine labeling and immunofluorescent staining patterns.

    Conclusions:

    • A significant reduction in cell surface LETS glycoprotein occurs during the establishment of continuous cell lines.
    • This decrease in LETS glycoprotein can happen without concurrent cellular transformation.
    • Findings clarify the role of LETS protein in restoring fibroblastic phenotype to transformed cells.