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Updated: Dec 10, 2025
Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
Published on: December 31, 2017
Analysis of bacterial communities of infected primary teeth in a Mexican population
E-K Salas-López1, S Casas-Flores, N-E López-Lozano
1Facultad de Estomatología Universidad Autónoma de San Luis Potosí Av. Dr. Manuel Nava #2, Zona Universitaria C.P. 78290, San Luis Potosí, S.L.P. México apozos@uaslp.mx.
Background:
The objective of this study was to describe the bacterial communities associated with pediatric patients with endodontic infections of temporal teeth by targeting the 16S rRNA gene using pyrosequencing.
Material And Methods:
Microbiological samples were obtained from the lower primary molars of thirteen 13 pediatric patients with dental infections. An aspiration method for microbiological sampling was used. The identification of microbiota employing the pyrosequencing method by targeting the 16S gene was performed.
Results:
Ribosomal 16S RNA gene sequences were amplified, obtaining a total of 16,182 sequences from 13 primary infected molars (13 different individuals) by pyrosequencing. Bacteroidetes phyla (35.15%) were the most abundant followed by Firmicutes (33.3%) and Fusobacteria (10.05%); the presence of specific pathogenic bacteria was determined as well.
Conclusions:
The infected root canal of primary teeth contains a high diversity of anaerobic bacteria, and Bacteroidetes, Firmicutes, and Fusobacteria phyla were the most abundant; Prevotella and Streptococcus genera were the most prevalent.
