Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nucleic Acid Structure01:25

Nucleic Acid Structure

8.1K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
8.1K
RNA Interference01:23

RNA Interference

27.5K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
27.5K
Nucleic Acids02:43

Nucleic Acids

48.8K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
48.8K
Nucleic acids02:43

Nucleic acids

187.0K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
187.0K
Riboswitches01:56

Riboswitches

9.3K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
9.3K
Experimental RNAi02:15

Experimental RNAi

7.0K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
7.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The power of triplets.

Nature reviews. Chemistry·2025
Same author

Engineering of Small Ribozymes Acting on RNA: What is Needed to Make a New Function Work with an Existing Catalyst?

Chembiochem : a European journal of chemical biology·2025
Same author

Nicotinamide Riboside: What It Takes to Incorporate It into RNA.

Molecules (Basel, Switzerland)·2024
Same author

Two RNA Folds from One Sequence: A Ribozyme with Versatile Substrate Processing Abilities.

Angewandte Chemie (International ed. in English)·2024
Same author

A New Architecture for DNA-Templated Synthesis in Which Abasic Sites Protect Reactants from Degradation.

Angewandte Chemie (International ed. in English)·2024
Same author

Stimuli-Responsive Boronate Formation to Control Nucleic Acid-Based Functional Architectures.

ChemPlusChem·2023

Related Experiment Video

Updated: Dec 10, 2025

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

1.7K

Reductive Charge Transfer through an RNA Aptamer.

Jennifer Frommer1,2, Sabine Müller1

  • 1Institute for Biochemistry, University Greifswald, Felix Hausdorff Str. 4, 17487, Greifswald, Germany.

Angewandte Chemie (International Ed. in English)
|August 28, 2020
PubMed
Summary

Charge transfer through RNA duplexes and non-Watson-Crick regions was investigated. This study shows distance-dependent reductive charge transfer in RNA, suggesting potential roles in RNA function.

Keywords:
RNA aptamerscharge transferfluorescence quenchingoptical spectroscopypyrene-modified RNA

More Related Videos

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

11.0K
An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
07:55

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

Published on: February 17, 2023

4.9K

Related Experiment Videos

Last Updated: Dec 10, 2025

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

1.7K
Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

11.0K
An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
07:55

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

Published on: February 17, 2023

4.9K

Area of Science:

  • Biophysics
  • Molecular Biology
  • Biochemistry

Background:

  • Charge transfer in double helical DNA is well-studied.
  • Charge transfer in RNA remains less understood, with limited data on homonucleotide sequences.
  • RNA's role in the 'RNA world' necessitates understanding charge transfer in functional RNA structures.

Purpose of the Study:

  • To investigate charge transfer mechanisms in RNA duplexes.
  • To explore electron transport through non-Watson-Crick base-paired regions in RNA.
  • To determine if charge transfer can be coupled with RNA function.

Main Methods:

  • Experimental investigation of reductive charge transfer through synthesized RNA duplexes.
  • Analysis of charge transfer across non-Watson-Crick base-paired regions within an RNA aptamer.
  • Distance-dependent measurements of electron transport efficiency.

Main Results:

  • Demonstrated distance-dependent reductive charge transfer through RNA duplexes.
  • Confirmed charge transfer occurs through non-Watson-Crick base-paired regions.
  • Provided evidence for charge transport capabilities in functional RNA motifs.

Conclusions:

  • RNA facilitates distance-dependent charge transfer, similar to DNA.
  • Electron transport is possible through diverse RNA structural elements, including non-canonical base pairs.
  • These findings support the potential for charge transfer to be integrated with RNA functions in biological systems.