Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cingulin, a new peripheral component of tight junctions.

S Citi1, H Sabanay, R Jakes

  • 1MRC Laboratory of Molecular Biology, Cambridge, UK.

Nature
|May 19, 1988
PubMed
Summary

Researchers identified cingulin, a novel protein in avian cells, crucial for the structure and function of tight junctions (Zonula occludens). This finding advances understanding of epithelial cell adhesion and barrier formation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development and application of a low-noise, high-speed optical detector module for carbon density fluctuation measurements on Wendelstein 7-X.

The Review of scientific instruments·2026
Same author

Measurement of Stark-split beam and carbon charge exchange emissions for simultaneous B-field and temperature/rotation analysis at DIII-D.

The Review of scientific instruments·2024
Same author

Enhancing Thomson scattering polychromator performance with multi-pass spectral filters.

The Review of scientific instruments·2024
Same author

Visible core spectroscopy at Wendelstein 7-X.

The Review of scientific instruments·2024
Same author

Design study of an edge current density diagnostic using new high-performance single-channel beam emission spectrometers at DIII-D.

The Review of scientific instruments·2022
Same author

Pedestal fluctuation measurements with charge exchange imaging at the DIII-D tokamak.

The Review of scientific instruments·2022

Area of Science:

  • Cell Biology
  • Epithelial Biology
  • Molecular Biology

Background:

  • Tight junctions (Zonula occludens) form a selective barrier in polarized epithelia, crucial for separating cellular compartments.
  • The molecular composition and architecture of tight junctions remain largely unknown.
  • ZO-1 is the first identified protein associated with zonula occludens.

Purpose of the Study:

  • To identify and characterize new components of the tight junctional complex.
  • To investigate the molecular architecture of avian epithelial cell junctions.

Main Methods:

  • Protein purification from avian brush-border cells.
  • Biochemical characterization of the purified protein (cingulin).
  • Immunofluorescence and immunoelectron microscopy using anti-cingulin antibodies.

Related Experiment Videos

Main Results:

  • A new acidic, heat-stable, and elongated protein named cingulin was identified and purified.
  • Cingulin was localized to the apical terminal web and endofacial surfaces of the zonula occludens in avian brush-border cells.

Conclusions:

  • Cingulin is a novel component of the avian tight junction complex.
  • The localization of cingulin suggests its role in the structural organization and function of the zonula occludens.