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Divergence of Root Microbiota in Different Habitats based on Weighted Correlation Networks
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Host selection shapes crop microbiome assembly and network complexity.

Chao Xiong1,2, Yong-Guan Zhu1,3, Jun-Tao Wang1

  • 1State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.

The New Phytologist
|August 28, 2020
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Summary
This summary is machine-generated.

Crop microbiome assembly is primarily determined by plant compartments and species, not location or fertilization. Host selection shapes microbial communities from soil to leaves, reducing diversity and complexity along the way.

Keywords:
co-occurrence networkscommunity assemblycompartment nichesfertilization practicesplant microbiomesplant-microbe interactionssoil-plant continuum

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Area of Science:

  • Microbiology
  • Ecology
  • Plant Science
  • Agricultural Science

Background:

  • Plant microbiomes are crucial for host health and agricultural productivity.
  • The ecological processes governing crop microbiome assembly remain incompletely understood.
  • Understanding microbiome assembly is key to sustainable agriculture and crop management.

Purpose of the Study:

  • To investigate bacterial community assembly across soil and plant compartments in a maize-wheat/barley rotation system.
  • To determine the influence of compartment niche, host species, site, and fertilization on crop microbiome structure.
  • To identify potential microbial sources and enrichment processes within the soil-plant continuum.

Main Methods:

  • Collected 684 samples from soil (rhizosphere, bulk soil) and plant niches (rhizoplane, root endosphere, phylloplane, leaf endosphere).
  • Analyzed bacterial communities in a maize-wheat/barley rotation system under varied fertilization at two sites.
  • Employed source tracking to infer microbial origins and enrichment pathways.

Main Results:

  • Microbiome assembly was predominantly driven by compartment niche and host species, overriding site and fertilization effects.
  • Host selection pressure increased sequentially from soil to endosphere, reducing bacterial diversity and network complexity.
  • Bacilli and Methylobacteriaceae were identified as key biomarker taxa for wheat/barley and maize, respectively.

Conclusions:

  • Crop microbiomes are primarily derived from soil and are filtered and enriched across plant compartments.
  • Host species exert a strong selective pressure, particularly in the leaf endosphere.
  • Findings provide empirical evidence for microbiome assembly dynamics, informing strategies for sustainable agriculture.