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An efficient Fourier ptychographic microscopy method based on optimized pattern of LED angle illumination.

Haifeng Mao1, Xiaohui Wu2, Jufeng Zhao1

  • 1Zhejiang Provincial Key Lab of Equipment Electronics, Hangzhou Dianzi University, Hangzhou 310018, PR China; School of Electronics and Information, Hangzhou Dianzi University, Hangzhou 310018, PR China.

Micron (Oxford, England : 1993)
|August 28, 2020
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Summary

Fourier ptychographic microscopy (FPM) efficiency is improved using an optimized LED angle illumination pattern. This method accelerates FPM image acquisition without compromising reconstruction quality, enhancing overall performance.

Keywords:
EfficiencyFourier ptychographic microscopyOptimizationPattern of angle illumination

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Area of Science:

  • Optics
  • Microscopy
  • Image Reconstruction

Background:

  • Fourier ptychographic microscopy (FPM) offers high resolution and wide field of view.
  • Conventional FPM suffers from low efficiency due to large image capture requirements.

Purpose of the Study:

  • To propose an efficient FPM method using optimized LED angle illumination.
  • To accelerate FPM image acquisition without sacrificing reconstruction quality.

Main Methods:

  • Analyzing LED, aperture, and sample positional relationships to determine spectrum range and distribution.
  • Utilizing image quality assessment to derive differential expressions for illumination strategies.
  • Designing a rhombus-based LED array illumination pattern for enhanced efficiency.

Main Results:

  • The proposed method effectively improves FPM efficiency.
  • Image reconstruction quality is maintained with the optimized illumination strategy.
  • Simulated and experimental results validate the method's effectiveness.

Conclusions:

  • Optimized LED angle illumination is a viable strategy to enhance FPM efficiency.
  • The rhombus-based illumination pattern accelerates FPM data acquisition.
  • This approach addresses the trade-off between capture quantity and efficiency in FPM.