Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Somites and axon guidance.

K W Tosney1

  • 1Department of Biology, University of Michigan, Ann Arbor 48109.

Scanning Microscopy
|March 1, 1988
PubMed
Summary
This summary is machine-generated.

Embryonic segmentation is not due to tissue differences in sclerotome halves. Instead, neural crest cells and axons likely use cell-cell contact or molecular cues for guidance.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Filopodial initiation and a novel filament-organizing center, the focal ring.

Molecular biology of the cell·2001
Same author

Identification of an invariant response: stable contact with schwann cells induces veil extension in sensory growth cones.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2000
Same author

Contact with isolated sclerotome cells steers sensory growth cones by altering distinct elements of extension.

The Journal of neuroscience : the official journal of the Society for Neuroscience·1999
Same author

Cellular interactions that guide sensory and motor neurites identified in an embryo slice preparation.

Developmental biology·1996
Same author

Somite strips: an embryo fillet preparation.

Methods in cell biology·1996
Same author

Embryo slices.

Methods in cell biology·1996
Same journal

Deposition of supercoiled DNA on mica for scanning force microscopy imaging.

Scanning microscopy·1996
Same journal

Relative intranuclear magnesium and phosphorus contents in normal and tumor cells of the human thyroid gland as revealed by energy-dispersive X-ray microanalysis.

Scanning microscopy·1996
Same journal

Preparation of cultured smooth muscle cells from human myometrium for X-ray microanalysis.

Scanning microscopy·1996
Same journal

Combined bronchoalveolar-vascular casting of the canine lung.

Scanning microscopy·1996
Same journal

Effects of castration upon the morphology of the accessory sex organs of the male rat--a scanning electron microscopy study.

Scanning microscopy·1996
Same journal

Scanning electron microscopy of the accessory sex glands of the adult male rat.

Scanning microscopy·1996
See all related articles

Area of Science:

  • Developmental biology
  • Neuroscience
  • Cell biology

Background:

  • Somites form repeating segments along the embryonic axis.
  • Somites influence the patterning of sensory and sympathetic ganglia and spinal nerves.
  • Neural crest cells and axons preferentially migrate to the anterior sclerotome.

Purpose of the Study:

  • To investigate tissue-level differences between anterior and posterior sclerotome in chick embryos.
  • To determine if these differences explain the directional preference of neural crest cells and axons.
  • To elucidate the mechanisms of embryonic segmentation.

Main Methods:

  • Scanning electron microscopy (SEM) was used to examine chick embryos.
  • Tissue organization, extracellular matrix density, cell density, shape, and orientation were analyzed.

Related Experiment Videos

  • Comparisons were made between anterior and posterior sclerotome regions.

    • Main Results:

    • Tissue organization differences between anterior and posterior sclerotome were insufficient to explain cell/axon preference.
    • Extracellular matrix density did not significantly differ at the dorsal sclerotome boundary.
    • No obvious barriers, cell density, shape, or orientation differences were observed prior to axon outgrowth.

    Conclusions:

    • Embryonic segmentation of neural crest cells and axons is not driven by gross tissue organization differences in the sclerotome.
    • Guidance likely involves direct cell-cell contact or response to diffusible molecular cues.
    • Further research should focus on molecular signaling and cell surface interactions.