Spectrophotometric determination of α-hydroxytropolone pK values: A structure-acidity relationship study

α-Hydroxytropolones (αHTs) have a wealth of biological activity owing to their ability to serve as metalbinding fragments for many therapeutically valuable dinuclear metalloenzymes. They also have the potential to exist in as many as 4 protonation states under aqueous acidic or basic conditions. The following details how UV absorption can be used to generate pK _a values on a series of αHTs. The studies also provide some knowledge into how the acidity and basicity change with some different functional groups. These studies thus provide new strategies and knowledge that could be valuable in leveraging αHTs as metal-binding fragments in drug-development pursuits.