Related Experiment Video
Updated: Dec 10, 2025
14:58
High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
Published on: March 5, 2022
4.6K
Differentially Expressed Homologous Genes Reveal Interspecies Differences of Paragonimus Proliferus based on
1School of Basic Medicine, Kunming Medical University, Kunming 650504, China.
Helminthologia
|August 29, 2020
Summary
The transcriptome of Paragonimus proliferus was sequenced, revealing 7393 differentially expressed genes compared to related species. Core genes involved in parasite biology were often expressed at lower levels in P. proliferus.
Area of Science:
- Molecular Biology
- Parasitology
- Genomics
Background:
- Paragonimus proliferus is increasingly found in Southeast Asia, but its molecular biology remains poorly understood.
- Limited knowledge exists regarding the transcriptome of P. proliferus, hindering comparative analysis with other Paragonimus species.
Purpose of the Study:
- To sequence the transcriptome of Paragonimus proliferus for the first time.
- To compare the P. proliferus transcriptome with four other Paragonimus species to identify homologous and differentially expressed genes.
- To explore interspecies molecular differences and potential biological roles of identified genes.
Main Methods:
- Transcriptome sequencing of Paragonimus proliferus.
- Comparative analysis of P. proliferus transcriptome against Paragonimus skrjabini, P. kellicotti, P. miyazakii, and P. westermani.
- Identification of homologous and differentially expressed genes, including core genes across all comparisons.
Main Results:
- 7393 significantly differentially expressed genes were identified between P. proliferus and the other four species.
- 49 core genes, implicated in transcription, metabolism, and parasitic functions, were differentially expressed across all comparisons.
- A majority of identified genes, including core genes, showed lower expression levels in P. proliferus.
Conclusions:
- The identified differentially expressed genes, particularly core genes, may explain biological distinctions between Paragonimus species.
- This study provides foundational molecular data for future research on Paragonimus proliferus biology and distribution.
- Lower expression of core genes in P. proliferus suggests unique regulatory mechanisms or adaptations.
More Related Videos
Related Concept Videos
01:57Gene Families
9.6K
Gene families consist of groups of genes proposed to have originated from a common ancestor. Typically these arise through events in which a gene or genes are mistakenly duplicated during cell division. Unlike their parent genes (which are subject to selection pressure to maintain function), these gene copies do not need to preserve their sequences and may evolve at a relatively faster rate.
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
9.6K
02:05Gene Evolution - Fast or Slow?
7.8K
The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
In contrast, regions which code...
7.8K
01:15Diversity of Protists I
693
Excavata is a diverse group of protists that includes both chemoorganotrophic and phototrophic species, with some thriving in anaerobic environments. Among the key groups within Excavata are diplomonads and parabasalids, which are flagellated protists that lack mitochondria and chloroplasts. These microorganisms typically inhabit anoxic environments, such as the intestines of animals, where they exist either symbiotically or as parasites, relying on fermentation for energy production. Some...
693
02:32Exon Recombination
3.9K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...
Exon shuffling follows “splice frame rules.” Each exon...
3.9K
02:24Ribosome Profiling
4.0K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.0K