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In vitro Induction of Human Dental Pulp Stem Cells Toward Pancreatic Lineages
Published on: September 25, 2021
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Alginate/Pluronic F127-based encapsulation supports viability and functionality of human dental pulp stem
Suryo Kuncorojakti1,2, Watchareewan Rodprasert2, Supansa Yodmuang3,4
1International Graduate Course in Veterinary Science and Technology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330 Thailand.
Journal of Biological Engineering
|August 29, 2020
Summary
Encapsulating human dental pulp-derived stem cells (hDPSC) in alginate (ALG) and alginate-pluronic F127 (ALGPA) preserves their viability and function. This offers a promising platform for future diabetes regenerative therapy.
Area of Science:
- Biomedical Engineering
- Stem Cell Biology
- Regenerative Medicine
Background:
- Current diabetes treatments have adverse effects, and existing regenerative therapies face limitations.
- Alternative insulin-producing cell (IPC) sources and transplantation platforms are needed.
Purpose of the Study:
- To evaluate alginate (ALG) and pluronic F127-coated alginate (ALGPA) encapsulation of human dental pulp-derived stem cell (hDPSC)-derived IPCs.
- To assess the viability and functionality of encapsulated hDPSC-derived IPCs for potential diabetes treatment.
Main Methods:
- hDPSC-derived IPCs were encapsulated using ALG and ALGPA.
- Viability, glucose and insulin diffusion, and expression of key pancreatic markers were assessed.
- Glucose-responsive C-peptide secretion was measured post-encapsulation.
Main Results:
- ALG and ALGPA maintained hDPSC viability and allowed essential molecule diffusion.
- Encapsulated cells sustained pancreatic marker expression and insulin production for at least 14 days.
- Functional analysis demonstrated glucose-responsive C-peptide secretion after 14 days.
Conclusions:
- ALG and ALGPA encapsulation effectively preserves hDPSC-derived IPC viability and function in vitro.
- These encapsulation methods present a viable transplantation platform for future clinical applications in diabetes therapy.
