[Detection of Campylobacter jejuni and C. coli in waste water]

For the detection of C. jejuni and C. coli in waste water the content of rifampicin in selective media was reduced from 10 micrograms/ml (Bolton and Robertson 1982) to 5 micrograms/ml and the content of polymymyxin B was increased from 0,25 microliter/ml (Skirrow 1977) to 2.5 micrograms/ml. On the Basis of this modification the isolation of C. jejuni and C. coli from waste water samples by membrane filter technique as well as by enrichment procedure was possible. Before inoculation of waste water samples the enrichment broth was preincubated at least 1 d at 37 degrees C under microaerophilic conditions. The Campylobacter test strains showed significant differences of growth between preincubated and non preincubated enrichment broth in the first hours of incubation.