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Updated: Dec 10, 2025
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
Published on: August 23, 2019
miR-597-3p inhibits invasion and migration of thyroid carcinoma SW579 cells by targeting RAB23
Hu-Ling Wen1, Zheng-Min Xu2, Shi-Yu Lin3
1Department of Nuclear Medicine, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, China. wenhl1982023@163.com.
Introduction:
Nowadays, it is indicated that miRNA was anomaly expressed in tumour. Previous studies have shown that miRNAs can regulate the proliferation, invasion, and migration of cancer cell-related processes. Meanwhile, current investigations show that RAB23 also plays an important role in cancer cell-related processes. But the potential mechanism remains unclear.
Material And Methods:
SW579 cells were selected and transfected with miR-597-3p mimics. Then the expression of miR-597-3p and RAB23 were measured by quantitative real-time polymerase chain reaction (PCR) and western blotting, respectively. Subsequently, the abilities of proliferation, invasion, and migration of SW579 cells were researched. For further study, the Luciferase reporter assay proved that miR-597-3p could target the expression of RAB23, and the proteins of invasion and migration were also measured to clear the mechanism.
Results:
After being transfected with miR-597-3p mimics, the expression of miR-597-3p was remarkably increased and RAB23 was significantly decreased. The abilities of proliferation, invasion, and migration also decreased significantly. The miRTarase Database predicated and Luciferase reporter assay proved that RAB23 was the target gene of miR-597-3p. The expression of matrix metalloproteinase (MMP)-2, MMP-9, and N-cadherin was down-regulated, and the expression of E-cadherin was up-regulated.
Conclusion:
miR-597-3p could reduce the proliferation, invasion and migration abilities of SW579 cells, which may be related to the targeted inhibition of RAB23 expression and down-regulation of the expression levels of MMP-2, MMP-9 and N-cadherin proteins of SW579 cells.
Insights
MicroRNA-597-3p (miR-597-3p) inhibits cancer cell proliferation, invasion, and migration by targeting RAB23. This study clarifies the mechanism by which miR-597-3p affects key proteins involved in cancer progression.
Area of Science:
- Molecular Biology
- Cancer Research
- Biochemistry
Background:
- MicroRNAs (miRNAs) are aberrantly expressed in tumors and regulate cancer cell processes.
- RAB23 is implicated in cancer, but its precise role and regulatory mechanisms remain unclear.
Purpose of the Study:
- To investigate the role of miR-597-3p in regulating cancer cell proliferation, invasion, and migration.
- To elucidate the underlying molecular mechanism involving RAB23.
Main Methods:
- SW579 cells were transfected with miR-597-3p mimics.
- Quantitative real-time PCR and Western blotting were used to measure gene and protein expression.
- Cellular proliferation, invasion, and migration assays were performed.
- Luciferase reporter assays confirmed the targeting of RAB23 by miR-597-3p.
Main Results:
- miR-597-3p mimics significantly increased miR-597-3p expression and decreased RAB23 expression.
- Cancer cell proliferation, invasion, and migration abilities were significantly reduced.
- RAB23 was validated as a direct target of miR-597-3p.
- Expression of MMP-2, MMP-9, and N-cadherin decreased, while E-cadherin increased.
Conclusions:
- miR-597-3p suppresses SW579 cell proliferation, invasion, and migration.
- This effect is mediated by the targeted inhibition of RAB23.
- Down-regulation of MMP-2, MMP-9, and N-cadherin, and up-regulation of E-cadherin contribute to the observed anti-cancer effects.
