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Related Experiment Video

Updated: Dec 10, 2025

Human Saphenous Vein Endothelial Cell Isolation and Exposure to Controlled Levels of Shear Stress and Stretch
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Endothelial microparticles induced by cyclic stretch activate Src and modulate cell apoptosis.

Fei Zhuang1, Han Bao1, Qian Shi1

  • 1Institute of Mechanobiology & Medical Engineering, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|August 29, 2020
PubMed
Summary

Mechanical stretch alters endothelial microparticles (EMPs), modulating endothelial cell (EC) apoptosis via Src activation. CD151 on EMPs is key in this EC communication and homeostasis process.

Keywords:
Srcapoptosiscyclic stretchendothelial microparticlesfluorescence resonance energy transfer

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Area of Science:

  • Cardiovascular Biology
  • Cellular Mechanotransduction
  • Endothelial Cell Biology

Background:

  • Endothelial microparticles (EMPs) are implicated in cardiovascular diseases.
  • Endothelial cells (ECs) face mechanical cyclic stretch (CS) from blood pressure.
  • The role of CS-induced EMPs in EC homeostasis remains largely unknown.

Purpose of the Study:

  • To investigate the impact of CS-induced EMPs on EC homeostasis.
  • To elucidate the molecular mechanisms by which CS-EMPs affect ECs.
  • To identify specific components within CS-EMPs involved in EC communication.

Main Methods:

  • Fluorescence resonance energy transfer (FRET) and western blotting to assess Src activation.
  • Proteomic analysis to compare EMP composition under different CS conditions.
  • Bioinformatic analysis to predict protein interactions.
  • Assessment of EC apoptosis and EMP-EC binding.

Main Results:

  • 5% CS-induced EMPs significantly increased Src activation in ECs.
  • Proteomic analysis revealed distinct EMP content differences between 5% and 15% CS groups.
  • CD151 on EMPs was identified as a key mediator, activating Src and enhancing EMP-EC binding.
  • CS-derived EMPs decreased EC apoptosis, an effect reversed by Src inhibition.

Conclusions:

  • Mechanical stretch modifies EMP composition, influencing EC apoptosis through Src activation.
  • CD151 expressed on CS-induced EMPs plays a critical role in EC communication and maintaining EC homeostasis.