Singlet Oxygen and Protochlorophyllide Detection in Arabidopsis thaliana

Since the recognition of the reactive oxygen species singlet oxygen (¹O₂) as a versatile signal that induces various stress responses, the mechanisms underlying ¹O₂-induced signaling transduction pathways have become the subject of much current research. This in turn highlights the need for reliable detection methods for ¹O₂. Here we describe a protocol for the detection of ¹O₂ using a commercially available fluorescent probe (Singlet Oxygen Sensor Green) and provide a simple method for direct visualization and quantification of the ¹O₂-evolving photosensitizer protochlorophyllide in the Arabidopsis fluorescent mutant.