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Oligosaccharide Assembly

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...

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Updated: Dec 10, 2025

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

Published on: February 5, 2018

Enzymatic Building-Block Synthesis for Solid-Phase Automated Glycan Assembly.

Andrea Marchesi¹, Fabio Parmeggiani^1,2, João Louçano³

¹Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester, UK.

Angewandte Chemie (International Ed. in English)

|August 29, 2020

Summary

Biocatalysis provides essential building blocks for automated oligosaccharide synthesis. This study demonstrates using a specific enzyme to create the LacNAc motif for automated assembly of complex glycans.

Keywords:

biocatalysis chemoenzymatic synthesis glycans oligosaccharides transgalactosylation

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Last Updated: Dec 10, 2025

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

Published on: February 5, 2018

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis

Published on: September 6, 2019

High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles

High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles

Published on: July 6, 2012

Area of Science:

Carbohydrate Chemistry
Glycobiology
Biocatalysis

Background:

Automated chemical synthesis of oligosaccharides is challenging due to the need for large quantities of protected building blocks.
Cell-surface glycans play crucial roles in biological processes, making their synthesis important for research.

Purpose of the Study:

To demonstrate the use of biocatalysis for generating activated building blocks for automated oligosaccharide synthesis.
To showcase the enzymatic synthesis of the N-acetyllactosamine (LacNAc) motif using a promiscuous galactosyltransferase.

Main Methods:

Utilized the promiscuous NmLgtB-B β1-4 galactosyltransferase from Neisseria meningitidis.
Employed lactose or sucrose as glycosyl donors for enzymatic synthesis.
Integrated the enzymatically produced LacNAc building block into automated oligosaccharide assembly.

Main Results:

Achieved fast and robust enzymatic synthesis of the LacNAc motif.
Demonstrated successful incorporation of the enzymatically synthesized LacNAc unit into a tetrasaccharide target via automated assembly.
Showcased the enzyme's ability to utilize common sugars as donors.

Conclusions:

Biocatalysis offers an efficient method for supplying key reagents for automated oligosaccharide synthesis.
Enzymatic generation of the LacNAc motif facilitates the construction of complex cell-surface glycan structures.
This integrated approach combines enzymatic and chemical methods for streamlined glycan synthesis.