Characterization and immune function of decapentaplegic (Dpp) gene from the oriental river prawn, Macrobrachium...

The Decapentaplegic (Dpp) gene, which belongs to the TGF-β superfamily, is involved in multiple developmental processes in eukaryotic species. In this study, we firstly identified and characterized Dpp from Macrobrachium nipponense. Its full-length open reading frame (ORF) cDNA was 1332 bp, encoding 443 amino acids. The putative MnDpp protein contained a signal peptide, a TGF-β propeptide region and a TGF-β domain. Its TGF-β domain was highly conserved from vertebrates to invertebrates, and exhibited highly similarity to Dpp derived from Bombyx mori. qRT-PCR analysis suggested that MnDpp expressed in all tested tissues and responded to both bacterial and virus pathogens, indicating MnDpp was involved in the innate immune response of M. nipponense. Knockdown of MnDppin vivo significantly increased bacteria growth and markedly decreased the expressions of NF-κB signaling genes including dorsal, relish, TAK1, TAB1, Ikkβ and Ikkε as well as antimicrobial peptides (AMPs) including ALF2, ALF3, ALF4, ALF5, Cru1 and Cru2. Moreover, in vitro overexpression of MnDpp protein in HEK293T cells further demonstrated that it exerted antibacterial immune response by activation of NF-κB signaling cascade. In summary, these results indicated that MnDpp played an important role in the innate immunity in M. nipponense by modulating NF-κB signaling pathway, which might provide new insights about Dpp in crustaceans and paved the way for a better understanding of the crustacean innate immune system.