Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunocytochemistry and sperm pathology.

B Baccetti1, A G Burrini, G Collodel

  • 1Centro di Studio per le Cellule germinali del Consiglio Nazionale delle Ricerche, Siena, Italy.

Journal of Submicroscopic Cytology and Pathology
|January 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intracardiac echocardiography in the diagnosis of prosthetic valve endocarditis.

BMJ case reports·2025
Same author

News on Sperm Evolution.

Development, growth & differentiation·2023
Same author

SA1/SA2 cohesion proteins and SIRT1-NAD+ deacetylase modulate telomere homeostasis in cumulus cells and are eligible biomarkers of ovarian aging.

Human reproduction (Oxford, England)·2018
Same author

Taste receptor polymorphisms and male infertility.

Human reproduction (Oxford, England)·2017
Same author

Protective effect of Propolfenol<sup>®</sup> on induced oxidative stress in human spermatozoa.

Andrologia·2017
Same author

Sperm with fibrous sheath dysplasia and anomalies in head-neck junction: focus on centriole and centrin 1.

Andrologia·2016
Same journal

The myofibroblast: a study of normal, reactive and neoplastic tissues, with an emphasis on ultrastructure.

Journal of submicroscopic cytology and pathology·2008
Same journal

Morphological, cytochemical, and ultrastructural study of thrombocytes and leukocytes in neotropical fish, Brycon orbignyanus Valenciennes, 1850 (Characidae, Bryconinae).

Journal of submicroscopic cytology and pathology·2007
Same journal

Mitochondrial encephalomyopathies: a review of routine morphological diagnostic methods with emphasis on the role of electron microscopy.

Journal of submicroscopic cytology and pathology·2007
Same journal

Expression of nestin in rat and human glomerular podocytes.

Journal of submicroscopic cytology and pathology·2007
Same journal

Beta-keratin localization in developing alligator scales and feathers in relation to the development and evolution of feathers.

Journal of submicroscopic cytology and pathology·2007
Same journal

Two types of autophagy in the podocytes in renal biopsy specimens: ultrastructural study.

Journal of submicroscopic cytology and pathology·2007
See all related articles

This study used immunocytochemistry to map key proteins in infertile human sperm. Protein distribution was linked to ultrastructure, revealing structural causes of male infertility.

Area of Science:

  • Reproductive Biology
  • Cell Biology
  • Spermatozoa Research

Background:

  • Male infertility affects a significant portion of the population.
  • Understanding the structural and molecular basis of sperm dysfunction is crucial for developing effective treatments.
  • Specific protein localization in spermatozoa is linked to their function and morphology.

Purpose of the Study:

  • To investigate the localization of acrosin, actin, tubulin, and vimentin in human infertile spermatozoa.
  • To correlate the distribution of these proteins with the ultrastructural characteristics of spermatozoa.
  • To elucidate the structural underpinnings of male infertility at the molecular level.

Main Methods:

  • Immunocytochemical techniques were employed for protein detection.

Related Experiment Videos

  • Ultrastructural analysis was performed on human spermatozoa.
  • Correlation analysis between protein distribution and sperm ultrastructure.

    • Main Results:

    • Significant differences in the localization patterns of acrosin, actin, tubulin, and vimentin were observed in infertile spermatozoa compared to controls (implied).
    • Specific protein mislocalization was associated with distinct ultrastructural defects.
    • The findings provide insights into the molecular basis of sperm morphological abnormalities.

    Conclusions:

    • The study clarifies the structural bases of infertility by linking protein localization to sperm ultrastructure.
    • Aberrant protein distribution is a key factor in the pathogenesis of male infertility.
    • This research contributes to a deeper understanding of sperm function and dysfunction.