Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Dec 10, 2025

Author Spotlight: Advancing Protein Engineering – Harnessing Evolution Through PRANCE and Lab Automation

Author Spotlight: Advancing Protein Engineering – Harnessing Evolution Through PRANCE and Lab Automation

Published on: January 12, 2024

Using phage-assisted continuous evolution (PACE) to evolve human PD1.

Xiaoxiao Ye¹, Min Tu¹, Mingxin Piao¹

¹Jilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, 5333 Xi'an Road, Changchun, 130062, China; Basic Forestry and Proteomics Research Center, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

Experimental Cell Research

|August 30, 2020

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Genome-Wide Identification, Evolution, and Expression Analysis of RING Finger Gene Family in <i>Solanum lycopersicum</i>.

International journal of molecular sciences·2019

Same author

LncRNA NEAT1 promotes autophagy via regulating miR-204/ATG3 and enhanced cell resistance to sorafenib in hepatocellular carcinoma.

Journal of cellular physiology·2019

Same author

Adverse drug reaction detection via a multihop self-attention mechanism.

BMC bioinformatics·2019

Same author

[Analysis of a pedigree with autosomal dominant intermediate Charcot-Marie-Tooth disease type E and nephropathy].

Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics·2019

Same author

Autophagy: A novel mechanism of chemoresistance in cancers.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2019

Same author

Novel mutations in DPM3 cause dystroglycanopathy with central nervous system involvement.

Clinical genetics·2019

Researchers evolved PD1 (Programmed cell death protein 1) using PACE to create improved antagonists for cancer immunotherapy. These evolved PD1 variants show higher affinity and specificity for PDL1, offering potential for more effective tumor treatments.

Area of Science:

Immunology
Molecular Biology
Biotechnology

Background:

The PD1/PDL1 pathway is crucial for regulating cancer immune responses.
Current PD1/PDL1 inhibitors, primarily monoclonal antibodies, show success but face challenges like response prediction and resistance.
There is a need for novel PD1/PDL1 antagonists with improved characteristics for cancer therapy.

Purpose of the Study:

To evolve PD1 variants with enhanced binding affinity and specificity for PDL1.
To explore the potential of these evolved PD1s as novel tools for cancer immunotherapy.

Main Methods:

Utilized phage-assisted continuous evolution (PACE) for protein evolution.
Performed interactome analysis to assess specificity.

Keywords:

Interaction PACE PD1 PDL1

More Related Videos

Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems

Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems

Published on: October 14, 2025

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Published on: December 14, 2019

Related Experiment Videos

Last Updated: Dec 10, 2025

Author Spotlight: Advancing Protein Engineering – Harnessing Evolution Through PRANCE and Lab Automation

Author Spotlight: Advancing Protein Engineering – Harnessing Evolution Through PRANCE and Lab Automation

Published on: January 12, 2024

Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems

Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems

Published on: October 14, 2025

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Published on: December 14, 2019

Main Results:

Successfully evolved PD1 variants with significantly higher binding affinity to PDL1.
Demonstrated increased specificity of the evolved PD1s for PDL1 through interactome analysis.

Conclusions:

The evolved PD1 variants represent promising candidates for next-generation cancer immunotherapies.
These engineered PD1 proteins could overcome limitations of current monoclonal antibody treatments.