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Updated: Dec 10, 2025
ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza
Published on: June 9, 2011
Simultaneous and accurate visual identification of chicken, duck and pork components with the molecular amplification
Panzhu Qin1, Jianguo Xu2, Li Yao2
1Engineering Research Center of Bio-process, MOE, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, PR China; Research Center for Biomedical and Health Science, School of Life and Health, Anhui Science & Technology University, Fengyang 233100, PR China.
Abstract:
We propose a visual strategy for simultaneous detection of multiple adulterated components in beef by integration of multiple polymerase chain reaction (mPCR) with the lateral flow strip (LFS). The primer sets for adulterated components are uniquely designed with different nucleic acid tags (NAT), enabling the amplicons with specific wobbled sequences at two opposite ends. The wobbled sequences will precisely hybridize with the pre-immobilized capture probes on T lines (T1, T2 and T3) and C line, contributing to the coloration of LFS. Taking advantages of extraordinary amplification efficiency of PCR and simplicity of LFS, common adulterated components including chicken, duck and pork can be easily detected with LOD as low as 0.01% (wt%), which is comparable to that of quantitative real-time polymerase chain reaction (qPCR) but with more simplified operations and reduced costs. The method can be extended to identification of other components by replacing the functional primer set. This method can be a useful candidate for meat quality control at the resource-limited setups.
