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Related Experiment Video

Updated: Dec 10, 2025

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome
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A noteworthy interface-targeting fluorescent probe for long-term tracking mitochondria and visualizing mitophagy.

Fang Liu1, Long Zhang2, Fei Li1

  • 1School of Pharmacy, Jiangxi Science & Technology Normal University, Nanchang, 330013, PR China.

Biosensors & Bioelectronics
|August 31, 2020
PubMed
Summary

Researchers developed a novel fluorescent probe, CVP, for tracking mitochondria in living cells and tissues. This interface-targeting probe offers high selectivity and long-term visualization without affecting mitochondrial function.

Keywords:
High fidelityInterface-targetingLong-termMitochondrial probeMitophagyTissue imaging

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Chemical Biology

Background:

  • Mitochondria are vital for cellular functions, and their dysfunction is linked to various human diseases.
  • Current methods for tracking and visualizing mitochondria long-term are limited, hindering research.
  • Mitochondrial interfacial architecture inspired a new probe design strategy.

Purpose of the Study:

  • To develop a novel fluorescent probe for high-selectivity, long-term mitochondrial tracking in live systems.
  • To investigate the efficacy of an interface-targeting model for designing mitochondrial probes.
  • To synthesize and characterize a new probe, (E)-4-(2-(7-(diethylamino)-2-oxo-2H-chromen-3-yl)vinyl)-1-dodecylpyridin-1-ium iodide (CVP).

Main Methods:

  • Design and synthesis of the CVP probe based on an interface-targeting model.
  • Utilizing a cationic pyridinium unit and a C12-chain for mitochondrial targeting.
  • Testing CVP's performance in live cells, tissues, and zebrafish for mitochondrial visualization and tracking.

Main Results:

  • CVP demonstrated excellent tissue and zebrafish permeability due to its C12-chain.
  • CVP achieved rapid and long-term mitochondrial staining, independent of mitochondrial membrane potential.
  • The probe exhibited high mitochondrial selectivity and low background noise, enabling visualization without washing.
  • CVP successfully visualized four types of mitochondria in tissues and tracked mitophagy in real-time.

Conclusions:

  • The interface-targeting model is effective for designing selective mitochondrial fluorescent probes.
  • CVP is a promising tool for studying mitochondrial dynamics, mitophagy, and related diseases.
  • The developed probe offers significant advantages over traditional mitochondrial tracking methods.