Multiflorane suppresses the proliferation, migration and invasion of human glioblastoma by targeting MAPK signalling pathway
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Published on: October 20, 2016
Peng Qiu1, Xiangrong Zheng, Hongan Yang
1Department of Neurosurgery, Shandong Provincial Hospital affiliated to Shandong University ， Jinan ， Shandong ， China, 250021.
Purpose:
Glioblastoma is one common malignant tumors of central nervous system and its treatment is limited by the unavailability of chemotherapeutic agents. This study was therefore undertaken to evaluate the anticancer effects of Multiflorane molecule against the U87 human glioblastoma cells and normal human astrocytes.
Methods:
The CCK8 assay was used to determine cell proliferation. Acridine orange (AO)/Ethidium bromide (EB) staining assays were used to detect apoptosis. Transwell assays were used to detect the cell migration and invasion. Western blotting was used to determine protein expression.
Results:
The results showed Multiflorane inhibited the proliferation of the human U87 cells with little effects on the normal cells. Investigation of the underlying mechanisms showed that Multiflorane induced apoptosis in U87 cells. Multiflorane-induced apoptosis was linked with upregulation of cleaved caspase-3, 8 and 9, as well as cleaved PARP. The Bax protein levels were increased and of Bcl-2 were decreased. Flow cytometric analysis showed that Multiflorane induced G2/M arrest of the U87 glioblastoma cells. Transwell assays showed that the molecule suppressed the migration and invasion of the U87 glioblastoma cells. Additionally, Multiflorane also blocked the phosphorylation of p38 MAPK1 dose-dependently.
Conclusion:
Taken together, Multiflorane may prove beneficial in the treatment of glioblastoma.
Insights
Multiflorane effectively inhibited glioblastoma cell proliferation and induced apoptosis. This molecule suppressed glioblastoma cell migration and invasion, showing potential as a glioblastoma treatment.
Area of Science:
- Neuro-oncology
- Molecular Biology
- Pharmacology
Background:
- Glioblastoma is a prevalent and aggressive central nervous system malignancy.
- Current glioblastoma treatments face limitations due to a lack of effective chemotherapeutic agents.
Purpose of the Study:
- To evaluate the anticancer effects of the Multiflorane molecule on U87 human glioblastoma cells.
- To assess the impact of Multiflorane on normal human astrocytes for safety evaluation.
Main Methods:
- Cell proliferation was assessed using the CCK8 assay.
- Apoptosis was detected via Acridine Orange/Ethidium Bromide staining.
- Cell migration and invasion were analyzed using Transwell assays.
- Protein expression and signaling pathways were investigated by Western blotting and flow cytometry.
Main Results:
- Multiflorane significantly inhibited U87 glioblastoma cell proliferation with minimal effects on normal astrocytes.
- Multiflorane induced apoptosis in U87 cells, evidenced by increased cleaved caspase-3, 8, 9, and PARP, and altered Bax/Bcl-2 ratios.
- The molecule caused G2/M cell cycle arrest and suppressed glioblastoma cell migration and invasion.
- Multiflorane dose-dependently inhibited p38 MAPK phosphorylation.
Conclusions:
- Multiflorane exhibits significant anticancer properties against glioblastoma cells.
- The findings suggest Multiflorane's potential as a therapeutic agent for glioblastoma treatment.
- Further research into Multiflorane's mechanisms and efficacy is warranted.
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