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Published on: July 7, 2015
Application of Molecularly Imprinted Polymer-modified Potentiometric Sensor for Quantitative Determination of
Atsuko Konishi1, Shigehiko Takegami2, Yoko Idosaki2
1Department of Analytical Chemistry, Kyoto Pharmaceutical University, 5 Misasaginakauchi-cho, Yamashina, Kyoto, 607-8414, Japan. konishi@mb.kyoto-phu.ac.jp.
Abstract:
A molecularly imprinted polymer-modified potentiometric histamine (HIS) sensor was prepared and used for quantitative determination of HIS in bovine serum. The calibration curve using the potential responses measured in 1 × 10-3 mol L-1 phosphate buffer (pH 7.4) showed good linearity in the HIS concentration range of 3 × 10-4 to 1 × 10-2 mol L-1 (r = 0.92), with a detection limit of 1.6 × 10-4 mol L-1. In bovine serum samples, the HIS sensor showed good recovery values of 91 - 104%. Therefore, this HIS sensor successfully determined the HIS concentration in bovine serum samples.
