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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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BODIPY-Hg2+ Complex: A Fluorescence "Turn-ON" Sensor for Cysteine Detection.

Monu Kumar1, Garima Chaudhary1, Amit Pratap Singh1

  • 1Department of Applied Sciences, National Institute of Technology Delhi, New Delhi, 110 040, India.

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
|September 1, 2020
PubMed
Summary

A novel BODIPY-based sensor, HLPy, selectively detects Hg2+ ions with high affinity and fluorescence quenching. This sensor also detects Cysteine (Cys) via a reversible mechanism, demonstrating reusability and potential for Hg2+ removal from water.

Keywords:
BODIPYHg2+ ions detectionJob’s plotcysteine

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Area of Science:

  • * Chemical Sensing and Molecular Recognition
  • * Materials Science and Nanotechnology

Background:

  • * Development of selective and sensitive chemosensors for heavy metal ion detection is crucial for environmental monitoring and biological studies.
  • * BODIPY-based fluorescent probes offer advantages like high quantum yields and photostability, making them attractive for sensing applications.

Purpose of the Study:

  • * To synthesize and characterize a novel BODIPY-based sensing material (HLPy) for the selective detection of Hg2+ ions.
  • * To investigate the sensing mechanism, including complex formation and fluorescence response.
  • * To explore the potential of the synthesized material for detecting other analytes, such as biothiols, and for environmental remediation.

Main Methods:

  • * Synthesis of a BODIPY derivative (HLPy) incorporating a 2-amino pyridine receptor.
  • * Spectroscopic characterization (fluorescence, UV-Vis) and binding studies (Job's plot) to determine affinity and complex stoichiometry.
  • * Investigation of the response of the Hg2+ complex to other biomolecules and assessment of reusability and Hg2+ removal efficiency.

Main Results:

  • * The synthesized HLPy selectively detected Hg2+ ions with a high association constant (ka = 2.04 × 105 M-1) and a low limit of detection (LOD) of 54 nM.
  • * Formation of a non-fluorescent 2:1 complex, [Hg(LPy)2], was confirmed, which selectively responded to Cysteine (Cys) with a 29 nM LOD through a reversible de-complexation mechanism and fluorescence recovery.
  • * The [Hg(LPy)2] complex demonstrated reusability for at least five cycles and successfully removed up to 89% of Hg2+ from aqueous samples.

Conclusions:

  • * The developed BODIPY-based sensor (HLPy) is effective for selective Hg2+ detection and exhibits a unique sensing capability for Cysteine.
  • * The reversible sensing mechanism and reusability of the Hg2+ complex highlight its potential for practical applications in environmental monitoring and biological sensing.
  • * The material shows promise for the remediation of mercury-contaminated water sources.