Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.0K
Atomic Force Microscopy01:08

Atomic Force Microscopy

4.2K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
4.2K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

660
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
660

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Thermo-mobility coupling: continuous knee temperature-step interaction patterns predict functional recovery after total knee arthroplasty.

European journal of orthopaedic surgery & traumatology : orthopedie traumatologie·2026
Same author

TweetyBERT: Automated parsing of birdsong through self-supervised machine learning.

Patterns (New York, N.Y.)·2026
Same author

Templated self-assembly of gold nanoparticles in smectic liquid crystals confined at 3D printed curved surfaces.

Nanoscale·2025
Same author

TweetyBERT: Automated parsing of birdsong through self-supervised machine learning.

bioRxiv : the preprint server for biology·2025
Same author

An automated platform for simultaneous, longitudinal analysis of engineered neuromuscular tissues for applications in neurotoxin potency testing.

Current research in toxicology·2025
Same author

Sacrificial capillary pumps to engineer multiscalar biological forms.

Nature·2024

Related Experiment Video

Updated: Dec 10, 2025

Author Spotlight: Modular Neuronal Networks for Analyzing Brain Functions
07:38

Author Spotlight: Modular Neuronal Networks for Analyzing Brain Functions

Published on: June 7, 2024

2.1K

Fast micron-scale 3D printing with a resonant-scanning two-photon microscope.

Benjamin W Pearre1, Christos Michas2, Jean-Marc Tsang2

  • 1Department of Biology, Boston University, Boston, MA 02215, USA.

Additive Manufacturing
|September 1, 2020
PubMed
Summary

A novel direct laser writing system uses a resonant mirror scanner for faster, micron-scale 3D printing. This open-source innovation makes high-resolution 3D printing accessible to two-photon microscope users.

Keywords:
3D printingAdditive manufacturingDirect laser writingLithographyResonant scanningTwo-photon microscopy

More Related Videos

Production of a Strain-Measuring Device with an Improved 3D Printer
06:17

Production of a Strain-Measuring Device with an Improved 3D Printer

Published on: January 30, 2020

6.4K
Stereolithographic 3D Printing with Renewable Acrylates
08:28

Stereolithographic 3D Printing with Renewable Acrylates

Published on: September 12, 2018

9.8K

Related Experiment Videos

Last Updated: Dec 10, 2025

Author Spotlight: Modular Neuronal Networks for Analyzing Brain Functions
07:38

Author Spotlight: Modular Neuronal Networks for Analyzing Brain Functions

Published on: June 7, 2024

2.1K
Production of a Strain-Measuring Device with an Improved 3D Printer
06:17

Production of a Strain-Measuring Device with an Improved 3D Printer

Published on: January 30, 2020

6.4K
Stereolithographic 3D Printing with Renewable Acrylates
08:28

Stereolithographic 3D Printing with Renewable Acrylates

Published on: September 12, 2018

9.8K

Area of Science:

  • Additive Manufacturing
  • Microfabrication
  • Optical Engineering

Background:

  • Direct laser writing (DLW) enables complex 3D object fabrication.
  • High-resolution DLW commonly utilizes femtosecond lasers and two-photon polymerization (2PP).
  • Current systems often rely on mechanical stages or galvanometers, limiting printing speed.

Purpose of the Study:

  • To develop a high-resolution 3D printing system with increased fabrication speed.
  • To adapt existing two-photon microscopy hardware for 3D printing applications.
  • To provide an accessible, open-source platform for micron-scale additive manufacturing.

Main Methods:

  • Modification of a commercial resonant-scanning two-photon microscope.
  • Implementation of a resonant mirror scanner for laser beam steering.
  • Development of integrated hardware control and software for the printing process.
  • Printing of micro-scale objects (approx. 400 × 400 × 350 μm).

Main Results:

  • Achieved printing speeds significantly higher than conventional DLW methods.
  • Maintained sub-micron resolution, characteristic of high-end 3D printing.
  • Demonstrated a complete, functional 3D printing process chain.
  • Validated performance through objective benchmarks.

Conclusions:

  • The resonant mirror scanner approach substantially increases DLW printing speed while preserving resolution.
  • Repurposing two-photon microscopes offers a cost-effective entry into micron-scale 3D printing.
  • The open-source release democratizes access to precision 3D printing technology for research and development.