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An Ex vivo Culture System to Study Thyroid Development
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An Ex vivo Culture System to Study Thyroid Development

Published on: June 6, 2014

Multihormonal regulation of thyroglobulin production by the OVNIS 6H thyroid cell line

A Aouani1, S Hovsépian, G Fayet

  • 1Laboratoire de Biochimie Médicale, Faculté de Médecine, Marseille, France.

Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Metabolisme
|February 1, 1988
PubMed

The hormonal regulation of thyroglobulin production has been studied using a clone of the ovine thyroid cell line: OVNIS 6H. 3 among the 6 hormones proposed for serum replacement are required for an optimal thyroglobulin production; insulin, hydrocortisone and thyrotropin. Insulin alone stimulates thyroglobulin production. The presence of insulin is also required to observe hydrocortisone and TSH stimulations. Newborn calf serum inhibits thyroglobulin production. The best conditions for optimal thyroglobulin expression and TSH responsiveness are obtained in serum-free medium supplemented with 5 micrograms/ml insulin, 100 nM hydrocortisone and 1 mU/ml TSH.

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