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Histocompatibility antigen changes associated with pink-eyed dilute (p) mutations
P J Wettstein1, V Chapman, E Birkenmeier
1Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
Immunogenetics
|January 1, 1988
Summary
The pink-eyed unstable (pun) mutation in mice is linked to a new histocompatibility antigen. This antigen is lost upon reversion to the wild-type coat color, demonstrating a connection between histocompatibility and coat color.
Area of Science:
- Immunogenetics
- Mammalian genetics
Background:
- The H-4 histocompatibility locus and pink-eyed dilute (p) locus are tightly linked.
- This linkage suggests a single gene may control both histocompatibility antigen and coat color.
Purpose of the Study:
- Investigate the effects of the pink-eyed unstable (pun) mutation on histocompatibility antigen phenotype.
- Determine if a single gene influences both coat color and histocompatibility.
Main Methods:
- Skin grafts from B6-pun substrains to C57BL/6 recipients.
- Transplantation of grafts from B6-pun donors to homozygous revertant (+/+) recipients.
- Analysis of graft rejection onset and intensity.
Main Results:
- Graft rejection observed at 6-7 weeks, but not complete destruction.
- Rejection onset and intensity were not accelerated by introducing new H-2 haplotypes.
- Grafts to revertant recipients showed crisis rejection, indicating a new histocompatibility antigen associated with the pun mutation.
Conclusions:
- The pun mutation is associated with a novel histocompatibility antigen.
- This antigen is lost upon reversion to wild-type.
- This study demonstrates the first link between histocompatibility and coat color phenotypes.