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Cloning of the gene, speB, for streptococcal pyrogenic exotoxin type B in Escherichia coli
G A Bohach1, A R Hauser, P M Schlievert
1Department of Microbiology, Medical School, University of Minnesota, Minneapolis 55455.
Infection and Immunity
|June 1, 1988
Abstract:
The structural gene encoding streptococcal pyrogenic exotoxin type B, designated speB, was cloned in Escherichia coli and localized onto a 4.5-kilobase BamHI-BglII DNA fragment. Streptococcal pyrogenic exotoxin type B, partially purified from E. coli clones, was immunologically related to streptococcus-derived toxin. Also, toxin derived from either E. coli or Streptococcus pyogenes had similar lymphocyte mitogenic activity and molecular weight (29,300) and displayed comparable microheterogeneity when evaluated by isoelectric focusing.