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Updated: Aug 19, 2026

Novel Production Protocol for Small-scale Manufacture of Probiotic Fermented Foods
Published on: September 10, 2016
Soymilk fermentation: effect of cooling protocol on cell viability during storage and in vitro gastrointestinal
Anna Carolina Meireles Piazentin1, Thamires Maria Simões da Silva1, Ana Carolina Florence-Franco2
1Department of Biochemical and Pharmaceutical Technology, University of São Paulo, Prof. Lineu Prestes, 580 - Bloco 16, Sao Paulo, 05508-000, Brazil.
Abstract:
This work covers soymilk fermentation by starter and probiotic cultures and explores the influence of cooling protocol on cell viability, organic acid production, sugar consumption, fatty acid profile, and cell survival to in vitro gastrointestinal stress. After fermentation at 37 °C by mono- or co-cultures of Streptococcus thermophilus (St), Lactobacillus bulgaricus (Lb), and Lactobacillus paracasei (Lp), fermented soymilk was cooled directly at 4 °C for 28 days or cooled in two phases (TPC), i.e., by preceding that step by another at 25 °C for 8 h. Soybean milk fermentation by Lb alone lasted longer (15 h) than by StLb or StLbLp (9 h). In ternary culture, TPC increased Lp viability, linoleic, and lactic acid concentrations by 3.8, 22.6, and 96.2%, respectively, whereas the cooling protocol did not influence Lp and St counts after in vitro gastrointestinal stress. Graphical abstract.
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