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Targeted Gene Silencing in Malignant Hematolymphoid Cells Using GapmeR
Atish Kizhakeyil1, Mobashar Hussain Urf Turabe Fazil2, Navin Kumar Verma2,3
1Lee Kong Chain School of Medicine, Nanyang Technological University, Singapore, Singapore. ATISH001@e.ntu.edu.sg.
Abstract:
Delivery of conventional antisense oligonucleotides or small interfering RNA (siRNA) molecules into hematolymphoid cells for targeted gene silencing has been proven to be difficult. Here, we describe a simple protocol to knockdown specific gene(s) in malignant hematolymphoid cells using "GapmeR." This protocol could be applicable to a wide range of cell-types and thus solves an important problem for researchers working with cell lines or primary cells derived from patients with hematolymphoid malignancies.
