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Identification of Enterobacteriaceae with the Minitek system
1Computer Identification Laboratory, Central Public Health Laboratory, London, UK.
The Journal of Applied Bacteriology
|February 1, 1988
Summary
This study evaluated the Minitek system for identifying Enterobacteriaceae and Vibrionaceae bacteria. The developed probability matrix improved identification accuracy, showing a low corrected error rate of 0.85%.
Area of Science:
- Clinical Microbiology
- Bacterial Identification
- Diagnostic Systems
Background:
- Accurate identification of bacterial pathogens is crucial for effective treatment.
- The Minitek system offers a method for bacterial identification, but its performance requires thorough evaluation.
Purpose of the Study:
- To assess the accuracy and reproducibility of the Minitek system for identifying Enterobacteriaceae and Vibrionaceae.
- To develop and validate an improved identification scheme using a probability matrix.
Main Methods:
- Examined 417 bacterial strains (361 Enterobacteriaceae, 56 Vibrionaceae) using Minitek system tests.
- Processed results through manufacturer-devised schemes and a novel probability matrix incorporating 35 tests.
- Assessed test reproducibility by testing 43 strains multiple times.
Main Results:
- Developed a probability matrix to enhance identification success.
- Determined the order of separating value for individual Minitek tests.
- Achieved a corrected error rate of 0.85% for strain identification.
Conclusions:
- The developed probability matrix significantly improves the accuracy of bacterial identification using the Minitek system.
- The Minitek system demonstrates good reproducibility for identifying Enterobacteriaceae and Vibrionaceae.